165,802 results

1. HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

   Plasmid

   #217737

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGR6 (ADGRG6 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-846

   Promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE TRE dCas9-VP64

   Plasmid

   #50916

   Purpose

   Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A nuclease-deficient

   Promoter

   TRE

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. HDAC4 Flag

   Plasmid

   #13821

   Purpose

   Mammalian expression of human histone deacetylase 4 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC4 (HDAC4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUltra-U6-gRNAs1-5

   Plasmid

   #178569

   Purpose

   Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Insert

   5 gRNA-tRNA array with U6 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Thy1-Brainbow-1.1 M

   Plasmid

   #18726

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG

   Plasmid

   #217739

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL1 (ADGRL1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-844

   Promoter

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LifeAct-LSSmGFP

   Plasmid

   #186534

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) (AAV PHP.eB)

   Viral Prep

   #191726-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) (#191726). In addition to the viral particles, you will also receive purified AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) plasmid DNA. Expression of SYFP2 in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Promoter

   Tags

   None

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

9. HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG

   Plasmid

   #217740

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL2 (ADGRL2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-830

   Promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-BFP-sesRNA-GFP-WPRE-pA

    Plasmid

    #192063

    Purpose

    Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for tdTom
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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