We narrowed to 95,395 results for: LAS

1. pMH4-SYN-ChR2-tdimer2RFP

   Plasmid

   #22772

   Depositor

   Thomas Oertner

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

   Insert

   ChR2-tdimer2RFP
   

   Use

   Tags

   ChR2-tdimer2RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBlue-hFX-HA(AscI)

   Plasmid

   #15404

   Depositor

   Michael Ristow

   Insert

   human frataxin cDNA (FXN Human)
   

   Use

   Tags

   AU1 and HA

   Expression

   Bacterial

   Mutation

   700 bp insert can be released by digestion with r…

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. hIRmut-GFP

   Plasmid

   #22397

   Depositor

   Joseph Bass

   Article

   Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

   Insert

   IRmut
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Leu 62 to Proline

   Promoter

   Available since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pFETCh_HHEX

   Plasmid

   #72353

   Purpose

   Donor vector for 3' FLAG tag of human HHEX

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   HHEX Homology Arms (HHEX Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASP Flag Rab32 CA

   Plasmid

   #44834

   Depositor

   Matthew Scott

   Insert

   Rab32 (Rab32 Fly)
   

   Use

   Drosophila

   Tags

   Flag

   Expression

   Insect

   Mutation

   Q79L (constitutive active)

   Promoter

   Available since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pST50Trc3-DHFRFLAG

   Plasmid

   #63987

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST50Trc4-DHFRCYD

   Plasmid

   #64005

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   CYD

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST50Trc2-DHFRFLAG

   Plasmid

   #63969

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMZ035

   Plasmid

   #66972

   Purpose

   Expresses zebrafish Chk1 mutant

   Depositor

   Michael Lampson

   Article

   Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

   Insert

   zebrafish Chk1
   

   Use

   Tags

   Expression

   Mutation

   mutated S256E, S280E, T292E, and S301E

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMZ030

    Plasmid

    #66970

    Purpose

    Expresses wild type zebrafish Chk1 with C-term GFP fluorescent tag

    Depositor

    Michael Lampson

    Article

    Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

    Insert

    wildtype zebrafish Chk1
    

    Use

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUASt- Crb intraGFP

    Plasmid

    #67525

    Purpose

    Expression of transmembrane and the intracellular domains of Crb with GFP.

    Depositor

    Marta Llimargas Casanova, Elisabeth Knust

    Insert

    crumbs (crb Fly)
    

    Use

    Drosophila

    Tags

    GFP

    Expression

    Insect

    Mutation

    aa97-2103 deleted (extracellular domain)

    Promoter

    hsp70

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. {Ud}RpL14.Dm

    Plasmid

    #53220

    Purpose

    {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescue

    Depositor

    Diethard Tautz

    Insert

    RpL14 (RpL14 Fly)
    

    Use

    Attb drosophila melanogaster transformation

    Tags

    Expression

    Mutation

    14 synonymous mutations introduced

    Promoter

    endogenous promoter

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV2021

    Plasmid

    #37842

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Phsp-4GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp-4

    Available since

    Dec. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSABAD331

    Plasmid

    #45609

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    TvoVMAC48
    

    Use

    Tags

    GB1-6

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV4a-hFJX1-Flag

    Plasmid

    #46931

    Purpose

    expression of human FJX1-FLAG in mammalian cells

    Depositor

    R.D. Beauchamp, Natasha Deane

    Insert

    FJX1 (FJX1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

16. RF-227-UB-BMX

    Plasmid

    #79186

    Purpose

    Expression of the C-terminal fragment of BMX that is endogenously generated by caspase cleavage in mammalian cells

    Depositor

    Richard Fahlman

    Insert

    BMX (BMX Human)
    

    Use

    Tags

    3x Flag and ubiquitin

    Expression

    Mammalian

    Mutation

    delta 1-242

    Promoter

    CMV

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GDAP2

    Plasmid

    #73259

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NM_001135589.2 (GDAP2 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

    Plasmid

    #73706

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451 and D143N

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pJK579

    Plasmid

    #72426

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites

    Depositor

    T. J. Kappock

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    4 silent mutations introduce unique internal SpeI…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4X3C mAGNET

    Plasmid

    #67954

    Purpose

    Lentiviral vector coding EGFP followed by 4x miR-128, 4x miR-221 and 4x miR-222 target sequences

    Depositor

    Xue Han, Wilson Wong

    Article

    Sayeg et al ACS Synth Biol. 2015 Apr 13.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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