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pLV-EF1A-PARP12-298-462-Hygro

Plasmid

#238098

Purpose

Expression of structurally predicted WWE domain of PARP12 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP12 (PARP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 298-462 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-RNF146-45-182-EGFP-Hygro

Plasmid

#238091

Purpose

Expression of structurally predicted WWE domain of RNF146 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146 (RNF146 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 45-182 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX1-1-175-EGFP-Hygro

Plasmid

#238093

Purpose

Expression of structurally predicted WWE domain of DTX1 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX1 (DTX1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-175 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX4-1-166-EGFP-Hygro

Plasmid

#238095

Purpose

Expression of structurally predicted WWE domain of DTX4 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX4 (DTX4 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-166 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pME TAEL2.0-5xC120-minpro (JDW 1109)

Plasmid

#224507

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL2.0-5xC120

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-TAEL (JDW 1324)

Plasmid

#224508

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLC047 - pL2_p2x35s-CrGLK-tMAS

Plasmid

#203906

Purpose

Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

CaMV 2x35S

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 WWE-PARP

Plasmid

#192548

Purpose

pENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)

Use

Gateway entry vector

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 PARP

Plasmid

#192549

Purpose

E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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p35S_EVDdi

Plasmid

#167120

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Mutation

Deleted intron from full length element

Promoter

CaMV 35S

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)

Plasmid

#110143

Purpose

Transformation and expression of Csy4 and cogDsRed proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Use

Binary vector

Tags

cogDsRed

Expression

Bacterial and Plant

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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NUAK2 gRNA (BRDN0001145484)

Plasmid

#77000

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK2 (NUAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK2 gRNA (BRDN0001144927)

Plasmid

#77001

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK2 (NUAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK2 gRNA (BRDN0001146174)

Plasmid

#77002

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK2 (NUAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK2 gRNA (BRDN0001147522)

Plasmid

#77003

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK2 (NUAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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YF-AIP

Plasmid

#61525

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with EYFP and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP and EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
CF-AIP

Plasmid

#61526

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP and ECFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pDGB3omega1_KanR_BastaR

Plasmid

#186426

Purpose

optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Inserts

KanR
BastaR

Use

Synthetic Biology; Binary vector for escherichia …

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

CaMV 35S and Pnos

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHCD1Dsol

Plasmid

#102955

Purpose

Mammalian expression of human soluble CD1D cDNA

Depositor

Gennaro De Libero, Lucia Mori

Article

Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

Insert

Human soluble CD1D cDNA (CD1D Human)

Tags

BirA

Expression

Mammalian

Mutation

Lacks the transmembrane region

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMAS

Plasmid

#123200

Purpose

binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS tMAS::rLUC-I::pMAS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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