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We narrowed to 31,809 results for: son

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Showing: 11241 - 11260 of 31809 results

  1. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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  3. (364) SB KRAB-SadCas9

    Plasmid
    #163022
    Purpose
    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)
    Depositor
    Tonia Rex
    Publication
    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mammalian codon-optimized SadCas9
    Use
    Transposon
    Tags
    KRAB and myc NLS
    Expression
    Mammalian
    Promoter
    TET-On
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163022-plasmid-map-sequence-id-320905

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  5. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161817-plasmid-map-sequence-id-320651

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  7. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid
    #154838
    Purpose
    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection
    Depositor
    Patrick Phillips
    Publication
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    synthetic guide::∆HYGR::unc-54 3' UTR
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    ∆HYGR is promoterless and encodes aa 60-341
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154838-plasmid-map-sequence-id-307904

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  8. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid
    #140222
    Purpose
    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140222-plasmid-map-sequence-id-296741

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  9. pT2Apuro-TRE-synPCB2.0

    Plasmid
    #139481
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    Use
    Tol2 transposon donor vector
    Expression
    Mammalian
    Promoter
    Tet responsive element
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    139481-plasmid-map-sequence-id-276016

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  10. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139088
    Purpose
    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T, R324L,S409I, E480K, E543D, M694I, E1219V
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139088-plasmid-map-sequence-id-273884

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  11. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid
    #136652
    Purpose
    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136652-plasmid-map-sequence-id-273328

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  12. BII-gR-BtW-eSpCas9

    Plasmid
    #133358
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Publication
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9
    Tags
    Flag-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133358-plasmid-map-sequence-id-269429

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  15. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid
    #114090
    Purpose
    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized MbCas12a
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114090-plasmid-map-sequence-id-230476

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  17. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid
    #91852
    Purpose
    Luciferase reporter for IL2RA enhancer (IGI-P0347)
    Depositor
    Jacob Corn
    Publication
    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Sequence scrambled from ch10:6094588-6094934
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    91852-plasmid-map-sequence-id-180044

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  18. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179545-plasmid-map-sequence-id-354761

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  19. PB-PE SpG

    Plasmid
    #179081
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  20. PB-PE xCas9

    Plasmid
    #172652
    Purpose
    All-in-one prime editor piggyBac transposon (xCas9)
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172652-plasmid-map-sequence-id-346754

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Showing: 11241 - 11260 of 31809 results