We narrowed to 30,758 results for: PLE;

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pNIC-Bio2_SMAC(delta(55))_9His

Plasmid

#197976

Purpose

E. coli expression of SMAC(delta(55))-9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Tags

9His

Expression

Bacterial

Mutation

delta (1-55)

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pfastbac/mBmal62-447

Plasmid

#47335

Purpose

mBmal fragment cloned into pfastbac and crystallized

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Mutation

aa 62-447

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pHygroR-GMRWhite

Plasmid

#165886

Purpose

Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE671

Plasmid

#153237

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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K8.1Pr-pGL4.16

Plasmid

#131037

Purpose

Firefly Luciferase reporter for K8.1 promoter from KSHV

Depositor

Britt Glaunsinger

Article

Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

Insert

KSHV K8.1 100 bp promoter

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pU1/Sm/3' Box_(GLuc)_INT

Plasmid

#68438

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-2xEGFP shRNA resistant

Plasmid

#37925

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion after aa248, TTCCTGT=>TCCCGGT

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-RCM vpx

Plasmid

#115812

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpx (AF349680)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-ElonginB

Plasmid

#29504

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Elongin B (ELOB Human)

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pSYN-VWF-039

Plasmid

#120292

Purpose

VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein

Depositor

Robert Peters

Article

Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8

Insert

VWF D1-D2-D'-D3-Fc

Tags

Fc

Expression

Mammalian

Mutation

C1099A/C1142A

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-FOP

Plasmid

#136796

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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SLC9A3R1_PDZ_2

Plasmid

#103933

Purpose

Protein expression and purification of SLC9A3R1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF1b

Plasmid

#67494

Purpose

Expression of shRNA targeting human ARF1b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF1 shRNA (ARF1 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
PDLIM7_PDZ_1

Plasmid

#103940

Purpose

Protein expression and purification of ENIGMA PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDLIM7 (PDLIM7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hH1

Plasmid

#102847

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_hH1

Use

Pcr template for stagr inserts

Promoter

U6

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM65893

Plasmid

#153216

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-VER9063 vpr

Plasmid

#115825

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (Ver 9063)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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