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pST50Trc3-STRDHFR

Plasmid

#63973

Purpose

Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Tags

Strep II (STR)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAT590

Plasmid

#68908

Purpose

NanoLuc expressed constiutively from PBT1311 and GH049 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAT780

Plasmid

#68917

Purpose

NanoLuc expressed constiutively from PAM2 and GH022 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT793

Plasmid

#68926

Purpose

NanoLuc expressed constiutively from PAM4 and GH078 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-LacZ30

Plasmid

#65028

Purpose

GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Tags

lacZ

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDSAT

Plasmid

#62290

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAT890

Plasmid

#68931

Purpose

Int7 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int7

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pXRT1

Plasmid

#63151

Purpose

Trp1 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Article

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial and Yeast

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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LIIIalpha F C-D

Plasmid

#54384

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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LIIIalpha F A-B

Plasmid

#54382

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLU

Plasmid

#64174

Purpose

harbours marker genes LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pXRnMX

Plasmid

#63153

Purpose

Nat1 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Article

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial and Yeast

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAT752

Plasmid

#68903

Purpose

NanoLuc expressed constiutively from PAM2, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT754

Plasmid

#68905

Purpose

NanoLuc expressed constiutively from PAM4, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAS404

Plasmid

#15978

Depositor

Eishi Noguchi

Article

Nakashima et al Genetics. 1999 Jul . 152(3):853-67.

Type

Empty backbone

Tags

HA

Expression

Yeast

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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BB1_L_23_syn_BsaI

Plasmid

#89915

Purpose

Linker to construct BB1 from PCR product with FS2 and FS3 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-HphMx

Plasmid

#122919

Purpose

LYS2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-NatMx

Plasmid

#122921

Purpose

LYS2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSTC1

Plasmid

#39241

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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LIIc F 1-2 RNAi

Plasmid

#54402

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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