We narrowed to 15,234 results for: invs

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pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

Plasmid

#123344

Purpose

Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

Plasmid

#123336

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-cpCerulean3-FLARE-AKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (G112P)

Plasmid

#135496

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

G112P

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (L156P)

Plasmid

#135497

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

L156P

Promoter

CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)

Plasmid

#135501

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (I52P)

Plasmid

#135486

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

I52P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (Y59P)

Plasmid

#135492

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y59P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (D61P)

Plasmid

#135493

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

D61P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (V67P)

Plasmid

#135494

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

V67P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (Y84P)

Plasmid

#135495

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (E166P)

Plasmid

#135498

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

E166P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S159AS197A

Plasmid

#131523

Purpose

Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Serine 159 to Alanine and Serine 197 to A…

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 NPm

Plasmid

#126593

Purpose

Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 1-65 and L329A/L331A in t…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 CCm

Plasmid

#126594

Purpose

Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 203-228 and siRNA resista…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pS6-N-hα2M-H6

Plasmid

#128496

Purpose

Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-A788) (A2M Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-33/myc-His

Plasmid

#127492

Purpose

Expresses myc-tagged _MTS NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-33 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-33

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-78/myc-His

Plasmid

#127493

Purpose

Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-78 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-78

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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289aa Met133, 138, 186Ileu ELL2

Plasmid

#127272

Purpose

Expresses 289aa human ELL2 with Met133, 138, 186Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)

Expression

Mammalian

Mutation

Met133, 138, 186 Ileu, cut with XbaI blunt fillin

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

Plasmid

#121804

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

Plasmid

#121808

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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