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pICE LYS2-URA3

Plasmid

#122890

Purpose

LYS2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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Geph UTR3m shRNA

Plasmid

#121071

Purpose

Negative control shRNA for plasmid 121070: shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

Depositor

Angel de Blas

Publication

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)

Plasmid

#115429

Purpose

Selection for in-frame fusion expression constructs

Depositor

Nicholas Ingolia

Publication

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Type

Empty backbone

Tags

T2A-SpHIS5 and λN

Expression

Yeast

Promoter

PGK1

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET-PF2050

Plasmid

#100721

Purpose

Expression of Pyrococcus furiosus PF2050

Depositor

Byung Il Lee

Publication

Han et al FEBS Lett. 2012 May 7;586(9):1384-8. doi

Insert

PF2050

Expression

Bacterial

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pUC:FCP:ShBle:FCP:EGFP

Plasmid

#85987

Purpose

Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

Depositor

Thomas Mock

Publication

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Inserts

ShBle
EGFP

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pNR163

Plasmid

#79544

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNR292

Plasmid

#79548

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

mTagBFP

Expression

Bacterial

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Smad7-C

Plasmid

#80895

Purpose

mammalian expression of Smad7 C terminal domain

Depositor

Aristidis Moustakas

Insert

Smad7 (Smad7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

aa 206-426

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pNR165

Plasmid

#79646

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR166

Plasmid

#79547

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR187

Plasmid

#79553

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx4

Plasmid

#74343

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx4. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 4 (Dlx4 Mouse)

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-cat

Plasmid

#69933

Purpose

Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Publication

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-cat

Use

Synthetic Biology

Expression

Bacterial

Promoter

PlLacO1

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1F11

Plasmid

#70851

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

msta

Use

Gateway Cloning

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pHL1940

Plasmid

#53044

Purpose

PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA

Depositor

Han Lim

Inserts

cstA
csrA

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pFAB806

Plasmid

#47849

Purpose

BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

lambda tR2 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO4

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1228

Plasmid

#11410

Depositor

Sung-Hou Kim

Publication

Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

Insert

eukaryotic translation initiation factor

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pICE ADE2-MET17

Plasmid

#122885

Purpose

ADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-LEU2

Plasmid

#122888

Purpose

LYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-HIS3

Plasmid

#122891

Purpose

TRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

Enlarge