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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 11281 - 11300 of 65391 results

  2. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68710-plasmid-map-sequence-id-123992

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  11. pET32a-LIC

    Plasmid
    #62310
    Purpose
    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis, S-tag, and Trx-Tag
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62310-plasmid-map-sequence-id-326570

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  13. pENTR-DMD-Donor

    Plasmid
    #60605
    Purpose
    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Insert
    Hygromycin resistant gene
    Use
    Knock-in donor vector
    Expression
    Mammalian
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    60605-plasmid-map-sequence-id-97115

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Showing: 11281 - 11300 of 65391 results