Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 21,861 results for: SPR

Clear keywords
Showing: 11281 - 11300 of 21861 results

  1. pHybr_r2a>mG3-srt-his

    Plasmid
    #124805
    Purpose
    HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG3
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124805-plasmid-map-sequence-id-244310

    Enlarge

  11. cBEST3

    Plasmid
    #234659
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and kasO17tss
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234659-plasmid-map-sequence-id-492747

    Enlarge

  12. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid
    #244844
    Purpose
    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification
    Depositor
    Jennifer Doudna
    Article
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    iCas12a
    Use
    CRISPR
    Tags
    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS
    Expression
    Bacterial
    Mutation
    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…
    Promoter
    T7
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244844-plasmid-map-sequence-id-486520

    Enlarge

  14. qTAG-C-Solo-mStayGold-PEX3

    Plasmid
    #227304
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the PEX3 locus. For peroxisome visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PEX3 (Addgene #227303)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PEX3 Homology Arms flanking a mStayGold Tag (PEX3 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227304-plasmid-map-sequence-id-453636

    Enlarge

  15. qTAG-C-Solo-mStayGold-H2BC11

    Plasmid
    #227330
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227330-plasmid-map-sequence-id-452124

    Enlarge

  16. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

    Enlarge

  17. qTAG-N-Solo-mStayGold-CEP135

    Plasmid
    #227287
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227287-plasmid-map-sequence-id-452057

    Enlarge

  19. qTAG-C-moxGFP-Puro-MAPRE1

    Plasmid
    #207794
    Purpose
    Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207794-plasmid-map-sequence-id-416380

    Enlarge

  20. qTAG-N-Puro-moxGFP-TUBA1B

    Plasmid
    #207767
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207767-plasmid-map-sequence-id-413793

    Enlarge

Showing: 11281 - 11300 of 21861 results