165,803 results

1. Tubulin-LSSmGFP

   Plasmid

   #186535

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   Tubulin

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GPR77-Tango

   Plasmid

   #66373

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR77 (C5AR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. TFORF1569

   Plasmid

   #141841

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF680 (ZNF680 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GLE1

   Plasmid

   #155625

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   GLE1 (GLE1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HMGA2+GFP

   Plasmid

   #25405

   Depositor

   Sean Morrison

   Article

   Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

   Insert

   high mobility group AT-Hook 2 (Hmga2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   polyadenylation signal lacking

   Promoter

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pCI-Myc3-HPS4

   Plasmid

   #133931

   Purpose

   Expresses 3xMyc-HPS4 construct in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

   Insert

   HPS4 (HPS4 Human)
   

   Use

   Tags

   3xMyc tag

   Expression

   Mammalian

   Mutation

   Glutamic acid 229 to Glycine; Valine 552 to Methi…

   Promoter

   CMV I.E.

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF-BOS-T7-CA150

   Plasmid

   #21924

   Depositor

   Mariano Garcia-Blanco

   Article

   Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

   Insert

   CA150 (TCERG1 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

8. TFORF1512

   Plasmid

   #144366

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX3-2 (NKX3-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRhoA2G

   Plasmid

   #40179

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1ORF43-3xFLAG

    Plasmid

    #130880

    Purpose

    Expresses human C1ORF43 with C-terminal 3xFLAG tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge