We narrowed to 73,658 results for: LAS

1. pLZ12Km2-P23R:TA:ffluc

   Plasmid

   #88900

   Purpose

   Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

   Depositor

   Thomas Proft

   Article

   Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

   Insert

   toxin-antitoxin + firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-O-FUW-Olig2

   Plasmid

   #30131

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   Olig2 (Olig2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM3 = ncs-1p::nCre

   Plasmid

   #24034

   Depositor

   Cori Bargmann

   Article

   Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

   Insert

   ncs-1 (ncs-1 Nematode)
   

   Use

   Cre/Lox

   Tags

   nCre

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-O-FUW-Zic1

   Plasmid

   #30132

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   Zic1 (Zic1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHG6

   Plasmid

   #188254

   Purpose

   Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

   Depositor

   Maggie Smith, Hong Gao

   Article

   Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   act1p/actII-orf4

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ARF1 (ARF1A-c001)

   Plasmid

   #110258

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ARF1 (ARF1 Human)
   

   Use

   Tags

   StrepII-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFG036

   Plasmid

   #137996

   Purpose

   cI repressor expression plasmid

   Depositor

   Sébastien Rodrigue

   Article

   Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

   Insert

   cI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Thermosensitive

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHG7

   Plasmid

   #188255

   Purpose

   Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection

   Depositor

   Maggie Smith, Hong Gao

   Article

   Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   act1p/actII-orf4

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4134100

   Plasmid

   #152050

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHis-003

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pJYDN3

    Plasmid

    #162456

    Purpose

    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBACgus4x/cdc37/LRRK2 1867-2186

    Plasmid

    #40364

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    HisTev

    Expression

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available since

    Oct. 2, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Use

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_LolT_C41S

    Plasmid

    #211789

    Purpose

    Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

    Depositor

    Yang Hai

    Article

    Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

    Insert

    lolt mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pE_Cre_lambdaCos

    Plasmid

    #173785

    Purpose

    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    Cre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    S102Y and S279Y- please see depositor comments

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDest eGFP CUGBP1

    Plasmid

    #61276

    Purpose

    Mammalian expression of EGFP-tagged human CUGBP1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    CUG-BP (CELF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHG5

    Plasmid

    #188253

    Purpose

    Integrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selection

    Depositor

    Maggie Smith, Hong Gao

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcNB2

    Plasmid

    #89242

    Purpose

    Resurfaced Cell-penetrating 5F7 nanobody

    Depositor

    Brian McNaughton

    Article

    Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

    Insert

    pcNB2 (5F7)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 4H4H

    Plasmid

    #51314

    Depositor

    Cheryl Arrowsmith

    Insert

    4H4H (EHMT1 4H4H, Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pUdO2c

    Plasmid

    #210231

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4133227

    Plasmid

    #152132

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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