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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Publication

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1REST_sh_seq2_WPRE

Plasmid

#127574

Purpose

shRNA to knock down RE1-silencing transcription factor (REST)

Depositor

Malin Parmar

Publication

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

shRNA for knock down of REST (REST Human)

Use

Lentiviral

Promoter

U6

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

Plasmid

#127659

Purpose

Over-express EGFP-NLS-FLAG-cGAS

Depositor

Nicolas Manel

Publication

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-NLS-Flag

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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GPI_2xmCherry

Plasmid

#127812

Purpose

Homo-dimer mCherry-mCherry expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

glycosylphosphatidylinositol-mCherry-mCherry

Expression

Mammalian

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBbB19k-GFP

Plasmid

#126135

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Publication

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDmelOR-mRho.V5.mER.hOr47a

Plasmid

#126478

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Publication

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or47a (Or47a Fly)
Orco (Orco Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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SLF063: Lenti-fCAG-jRGECO1a

Plasmid

#124775

Purpose

For general lentiviral or direct expression of cytosolic jRGECO1a

Depositor

Adam Cohen

Publication

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

jRGECO1a

Use

Lentiviral

Tags

NES-6xHIS

Expression

Mammalian

Promoter

CAG

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSEVA6213S

Plasmid

#122094

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Promoter

Pem7

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB-Rex

Plasmid

#122134

Purpose

B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

Depositor

Srivatsan Raman

Publication

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

B. subtilis Rex

Expression

Bacterial

Promoter

apFAB338

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZ143-pcDNA3-BvCas12b

Plasmid

#122445

Purpose

Expresses BvCas12b in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BvCas12b-NLS-3xHA

Tags

3XHA

Expression

Mammalian

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hCdt1-HA

Plasmid

#117500

Purpose

Expresses human Cdt1 fused to HA tag

Depositor

Domenico Maiorano

Publication

Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

Insert

Cdc10-dependent transcript-1 (CDT1 Human, 1700 bp)

Tags

HA

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAPM-D4- miR30-L1221

Plasmid

#115846

Purpose

Control shRNA KD vector

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

control shRNA

Use

Lentiviral

Promoter

SFFV

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-ExRai-AktAR(T/A)

Plasmid

#118463

Purpose

Negative-control mutant for ExRai-AktAR biosensor.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-NLS-actin-R62D

Plasmid

#118381

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS and R62D

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-actin

Plasmid

#118378

Purpose

Gateway compatible donor vector with WT actin.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-155

Plasmid

#118537

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMRE-Tn7-146

Plasmid

#118562

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Publication

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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