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Plasmid#165564PurposeConnects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.DepositorInsertE. coli DNA Ligase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFGL821
Plasmid#58223PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL821-8
Plasmid#58224PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL822
Plasmid#58225PurposeFor fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.DepositorInsertphosphinothricin acetyl transferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL822-8
Plasmid#58226PurposeFor fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.DepositorInsertphosphinothricin acetyl transferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEC1.2/EC1.1_spCas9_GFP
Plasmid#161933PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC1.2/EC1.1_spCas9_TagRFP
Plasmid#161934PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGL1009
Plasmid#119080PurposeIntroducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).DepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFPT7
Plasmid#28012DepositorInsertT7 RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCFP
Plasmid#28011DepositorInsertCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFRm43GW
Plasmid#133748PurposeThree-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA
Plasmid#48884PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFG7m34GW
Plasmid#133747PurposeThree-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGL820
Plasmid#58221PurposeFor fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.DepositorInsertILV2
UseTagsExpressionBacterialMutationPromoterILV2 native promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL820-2
Plasmid#58222PurposeFor fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.DepositorInsertILV2
UseTagsExpressionBacterialMutationPromoterILV2 native promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW004
Plasmid#131805PurposeVector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'DepositorArticleInsertZea mays FNR and Z. mays SIR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGRG25
Plasmid#16665DepositorInsertmTn7::MCS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGRG36
Plasmid#16666DepositorInsertmTn7::MCS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Tq2CFP
Plasmid#136981PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only