We narrowed to 2,007 results for: endog

1. pcDNA3.1(+)-GFP11-RhoA

   Plasmid

   #181860

   Purpose

   Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   GFP11-RhoA (RHOA Human)
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pODN-H2BC11-TagBFP

   Plasmid

   #183867

   Purpose

   Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mTagBFP

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-HypaCas9-mR2-ECT2_sgRNA

   Plasmid

   #183875

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2A-mTurquoise-CDC42-G12V-deltaCAAX

   Plasmid

   #176094

   Purpose

   Constitutively active, nuclear localized Cdc42

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   CDC42 (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Hmga2 shMut m7

   Plasmid

   #52037

   Purpose

   encodes shMut HMGA2 m7

   Depositor

   Julian Downward

   Article

   Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   shRNA binding site mutated, all seven let-7 sites…

   Promoter

   CMV

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-Hmga2 shMut ATG wt

   Plasmid

   #52038

   Purpose

   encodes shMut HMGA2 ATG wt

   Depositor

   Julian Downward

   Article

   Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   shRNA binding site mutated, first ATG mutated

   Promoter

   CMV

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-Hmga2 shMut ATG m7

   Plasmid

   #52039

   Purpose

   encodes shMut HMGA2 ATG m7

   Depositor

   Julian Downward

   Article

   Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   shRNA binding site mutated, first ATG mutated, al…

   Promoter

   CMV

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. JA1280

   Plasmid

   #49969

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD HB-10 (HBB Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. JA1272

   Plasmid

   #49967

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD HB-8 (HBB Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. JA1266

    Plasmid

    #49966

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-7 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. JA1229

    Plasmid

    #49955

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-2 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. JA1217

    Plasmid

    #49954

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-1 (HBB Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. qTAG-N-Blast-mNeon-ACTB

    Plasmid

    #207751

    Purpose

    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEmax_AAVS1_knockin_HDR_donor

    Plasmid

    #223000

    Purpose

    HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PEmax
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid

    #187952

    Purpose

    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-CasRx-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker, HA-tag, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid

    #227325

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid

    #220349

    Purpose

    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

    Depositor

    Tomoko Igawa

    Article

    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

    Inserts

    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    

    Use

    Tags

    Superman Repression Domain X (SRDX) and VP16 tran…

    Expression

    Plant

    Mutation

    two amino acid substitution in WUS-box: L255A L25…

    Promoter

    CaMV 35S and RPS5A

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-N-Puro-mStayGold-LMNB1

    Plasmid

    #227329

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Human, Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Blast-dTAG-LMNB1

    Plasmid

    #207776

    Purpose

    Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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