We narrowed to 83,047 results for: ELL

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

Plasmid

#107730

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

Plasmid

#107729

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Klf4-pA-pgk-hph

Plasmid

#74906

Purpose

Mammalian expression of mKlf4

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Klf4 (Klf4 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

Plasmid

#123337

Purpose

Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Lyn-Venus-cpVenus-FLARE-AKAR

Tags

N-terminal targeting sequence from Lyn kinase, Ve…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-XRN1__PRKCE

Plasmid

#116846

Purpose

Lentiviral expression of XRN1__PRKCE fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

XRN1__PRKCE

Use

Lentiviral

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(259-443)

Plasmid

#89832

Purpose

Express GATA3 C1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C1 (AA 259-443)

Promoter

CMV

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-CKAR

Plasmid

#123343

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

Plasmid

#175423

Purpose

Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 1-117 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

nsp1 C-terminus

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 118-180 nsp1

Plasmid

#175424

Purpose

Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 118-180 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminus of nsp1

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Pf92-bio

Plasmid

#47728

Purpose

Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised Pf92

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDDB2-R273H-V5

Plasmid

#167469

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

R273H

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-mM₃O-IP

Plasmid

#46644

Depositor

Nobuaki Kikyo

Article

Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

Insert

mM₃O (Pou5f1 Mouse)

Use

Retroviral

Tags

IRES Puro and mouse MyoD (aa 1-62)

Expression

Mammalian

Mutation

contains a fragment of mouse MyoD (aa1-62) fused …

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-VSTM2L-COMP5AP-AviTag-9xHis

Plasmid

#157146

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSTM2L (VSTM2L Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-NTRK3__RBPMS

Plasmid

#116829

Purpose

Lentiviral expression of NTRK3__RBPMS fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NTRK3__RBPMS

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

Plasmid

#175417

Purpose

Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R124A/K125A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDR1__PAK1

Plasmid

#116813

Purpose

Lentiviral expression of DDR1__PAK1 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDR1__PAK1

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3G-COMP5AP-AviTag-9xHis

Plasmid

#157539

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3G (SEMA3G Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2K930R-FF-VSV

Plasmid

#139348

Purpose

Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-K930R-Y1054F-Y1055F

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK21054-5FF-VSV

Plasmid

#139347

Purpose

Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Expression

Mammalian

Mutation

V362F-Y1054F-Y1055F

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMP108 Nanog-Venus-2a-mCherry

Plasmid

#159743

Purpose

Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

Depositor

Sergi Regot

Article

Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

Insert

Nanog homeobox (Nanog Mouse)

Use

Mouse Targeting

Tags

Venus-2a-mCherry

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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