Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 29,279 results for: Tat

Clear keywords
Showing: 11461 - 11480 of 29279 results

  4. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Suzanne Paradis
    Article
    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do
    Insert
    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. GFP-DroshaS300E/S302D

    Plasmid
    #62531
    Purpose
    Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid
    Depositor
    Bharat Ramratnam
    Article
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Insert
    human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serine 300 to glutamic acid and serine 3…
    Available Since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. mCherry-CRY2-INPP5E

    Plasmid
    #79561
    Purpose
    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid
    #99694
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    99694-plasmid-map-sequence-id-194587

    Enlarge

  11. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid
    #122985
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains A58V,R73Q "milli" mutatio…
    Promoter
    human synapsin
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122985-plasmid-map-sequence-id-238429

    Enlarge

  12. pMXs-E55A-ATPIF1

    Plasmid
    #85404
    Purpose
    Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase
    Depositor
    David Sabatini
    Article
    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1
    Insert
    ATPIF1 (ATP5IF1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    E55A point mutation abrogating the ability of ATP…
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

    Enlarge

Showing: 11461 - 11480 of 29279 results