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pMAD-Gate2

Plasmid

#25941

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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PEP113E

Plasmid

#97399

Purpose

binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

Peroxisome target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHisIHFαIHFβ

Plasmid

#149384

Purpose

Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Inserts

Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta

Tags

6xHis tag and HRV3C cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pStrepIHFb

Plasmid

#149385

Purpose

Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta

Tags

HRV3C cleavage site and StrepII-tag with

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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PEP103E

Plasmid

#97389

Purpose

binary construct of plastid-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plastid target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PEP105E

Plasmid

#97391

Purpose

binary construct of peroxisome-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Peroxisome target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Vrg4-V5-APEX2-URA

Plasmid

#207071

Purpose

Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Vrg4

Tags

V5-APEX2

Expression

Yeast

Promoter

pVRG4

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC15-P1B3

Plasmid

#202856

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC15

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC11-P1A11

Plasmid

#202852

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC11

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJV0010

Plasmid

#205132

Purpose

Expression of 1xPX-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

1xPX domain of SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR29_00091361

Plasmid

#121209

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR29_00091361

Use

Golden gate

Mutation

Magnaporthe grisea

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pRPa-iSL(MCS1+2)

Plasmid

#69244

Purpose

Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

Depositor

David Horn

Article

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Promoter

RRNA promoter

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBORF-DBD

Plasmid

#127463

Purpose

ORF filtering plasmid (lambda cI associated plasmid for pAVA)

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIK-GW

Plasmid

#126556

Purpose

Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatible

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD

Plasmid

#127785

Purpose

Expresses CtCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain)

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBORF-AD

Plasmid

#127462

Purpose

ORF filtering plasmid (RNAp associated plasmid for pAVA)

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSpyTag-GlpG

Plasmid

#79054

Purpose

Expresses SpyTagged GlpG protein

Depositor

James Bowie

Article

Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

Insert

SpyTag-GlpG (glpG )

Tags

6xHis and SpyTag

Expression

Bacterial

Mutation

GlpG protein employed here corresponds to residue…

Promoter

Trc

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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