We narrowed to 13,071 results for: lic

1. pMPO 52

   Plasmid

   #68359

   Purpose

   Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII, ColE1 replication origin

   Depositor

   Eduardo Santero

   Article

   Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PrbC5

   Plasmid

   #24787

   Depositor

   David Baker

   Article

   Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

   Insert

   Prb
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

   Available Since

   July 11, 2011

   Availability

   Academic Institutions and Nonprofits only

3. BS.YxiN.Twin1.a1-368.wt

   Plasmid

   #13528

   Depositor

   David McKay

   Article

   Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

   Insert

   B. subtilis DEAD-box helicase YxiN first two domains
   

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   deleted amino acid residues 369-479

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. BS.YxiN.Twin1.a404-479.wt

   Plasmid

   #13529

   Depositor

   David McKay

   Article

   Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

   Insert

   B. subtilis DEAD-box helicase YxiN specific RNA binding domain
   

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   delete amino acid residues 1-403

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xHA-TurboID-NLS_pCDNA3

   Plasmid

   #107171

   Purpose

   expresses 3xHA-tagged TurboID in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MCS-BioID2-HA

   Plasmid

   #74224

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

   Plasmid

   #164514

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB402SC

   Plasmid

   #224552

   Purpose

   Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

   Depositor

   Paul Schulze-Lefert

   Article

   Lawson et al bioRxiv 2024.07.14.603419

   Type

   Empty backbone

   Tags

   Twin-Strep-HA

   Expression

   Plant

   Promoter

   2 X 35S

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB402SN

    Plasmid

    #224551

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Tags

    HA-Twin-Strep

    Expression

    Plant

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. V5-TurboID-NES_pCDNA3

    Plasmid

    #107169

    Purpose

    expresses V5-tagged TurboID in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23105 and tet

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)

    Plasmid

    #230015

    Purpose

    Expresses anti-TNFalpha PAGER(Gq) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-Ozoralizumab-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    W442A on hM1D

    Promoter

    CMV

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. His6-MBP-uTEV3

    Plasmid

    #135464

    Purpose

    Bacterial expression of uTEV3 (full-length) protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    His6-MBP-uTEV3
    

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability) , I138T/S153N…

    Promoter

    T5

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX304 CMV FKBP-V5-sTurboID (N)

    Plasmid

    #153002

    Purpose

    Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

    Plasmid

    #153003

    Purpose

    Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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