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We narrowed to 14,060 results for: crispr grnas

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Showing: 11561 - 11580 of 14060 results

  5. pX458-tRNA-SpCas9

    Plasmid
    #195183
    Purpose
    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.
    Depositor
    Stephanie Roessler
    Insert
    Cas9
    Use
    CRISPR
    Promoter
    tRNA-Gln
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195183-plasmid-map-sequence-id-423143

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  6. pCas12f1

    Plasmid
    #171182
    Purpose
    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1
    Depositor
    Kohsuke Honda
    Article
    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124
    Insert
    Cas12f1
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    cas9 promoter from Streptococcus pyogenes
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171182-plasmid-map-sequence-id-336407

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  7. pBHM2681

    Plasmid
    #241726
    Purpose
    Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source
    Depositor
    Hiten Madhani
    Article
    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Sept. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241726-plasmid-map-sequence-id-479856

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  10. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  11. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  12. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  13. pWR63

    Plasmid
    #203331
    Purpose
    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203331-plasmid-map-sequence-id-409751

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  14. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  15. pSJX016

    Plasmid
    #232324
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Promoter
    PJ23119 and Pvan
    Available Since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232324-plasmid-map-sequence-id-476126

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  16. pSJX023

    Plasmid
    #232326
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Promoter
    PJ23119 and Pxyl
    Available Since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    232326-plasmid-map-sequence-id-496249

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  17. pSJX022

    Plasmid
    #232325
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Promoter
    PJ23119 and Pvan
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232325-plasmid-map-sequence-id-478586

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  18. pSJX024

    Plasmid
    #232327
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Promoter
    PJ23119 and Pxyl
    Available Since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232327-plasmid-map-sequence-id-476136

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  19. pSJX067

    Plasmid
    #232328
    Purpose
    Genome editing in Agrobacterium fabrum
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Promoter
    PJ23119 and Pvan
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232328-plasmid-map-sequence-id-478585

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Showing: 11561 - 11580 of 14060 results