We narrowed to 6,774 results for: Ado

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pCDF1-MCS2-EF1-Puro-RGS7-E383K

Plasmid

#113731

Purpose

Express mutant RGS7- E383K- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

E383K

Promoter

CMV

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-R44C

Plasmid

#113729

Purpose

Express mutant RGS7- R44C- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-R310_RASA2-EF1-Puro

Plasmid

#114097

Purpose

Express mutant RASA2 in mamalian cells

Depositor

Yardena Samuels

Article

Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

Insert

RASA2 (RASA2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R310*

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-GFP-PGK-Puro

Plasmid

#110862

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRNA-Control

Plasmid

#98398

Purpose

Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

non-targeting randomized sequence

Use

Lentiviral

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV1, AAV5, and AAV8

Inserts

Caspase 3 (CASP3 Synthetic, Human)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTubb3-MC

Plasmid

#87112

Purpose

minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFP

Use

CRISPR

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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V5-ERG-R367K

Plasmid

#118624

Purpose

Expresses V5 tagged ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (R367K DNA binding mutant) (ERG Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R367K

Promoter

EF1a

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-PLXNB2-shRNA1

Plasmid

#98399

Purpose

Lentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-KIF1B beta-FL

Plasmid

#79108

Purpose

Mammalian expression of GFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta full length (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pBABE-hygro-2xMyc-Rac1-P29S

Plasmid

#128581

Purpose

For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Proline29 to Serine.

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-Q61L

Plasmid

#128582

Purpose

For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Glutamine61 to Leucine.

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAi14-GFPNLS-MC

Plasmid

#87114

Purpose

minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFPNLSPpA

Use

CRISPR

Promoter

nEF

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDHa_mDDX5_FL_wt

Plasmid

#88869

Purpose

constitutive expression of Ha-DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (Ddx5 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304 ERG R367K

Plasmid

#118626

Purpose

Overexpresses ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG ( R367K DNA Binding mutant) (ERG Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

R367K

Promoter

CMV

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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RFP-KIF1B beta-FL

Plasmid

#79111

Purpose

Mammalian expression of RFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

RFP-KIF1B beta full length (KIF1B Human)

Tags

RFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-6p1-GST-ArcΔCTD

Plasmid

#119878

Purpose

Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Tags

GST

Expression

Bacterial

Mutation

Deletion of nt 832-1110

Promoter

tac

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAi14-luc-MC

Plasmid

#87113

Purpose

minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

Luciferase-SV40pA

Use

CRISPR and Luciferase

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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