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pLL3.7-UBCp-hMGT-Ef1a-puro

Plasmid

#172396

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-dCas9-T2A-GFP

Plasmid

#193781

Purpose

Tricistronic expression of dCas9-EGFP-BSD

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

dead Cas9

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

In frame with existing Cas9 promoter

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMG046 pETARA-CK1α-His

Plasmid

#154074

Purpose

Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CSNK1A1 (CSNK1A1 Human)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti

Plasmid

#239610

Purpose

Constitutive active CRISPRgenee construct with a blasticidin resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

Plasmid

#239608

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnao1-A

Plasmid

#245998

Purpose

Express HA N-terminal tagged mouse GalphaoA transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnao1 (Gnao1 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaq

Plasmid

#246001

Purpose

Express HA N-terminal tagged mouse Galphaq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnaq (Gnaq Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.18 NR0B1-Luc

Plasmid

#100983

Purpose

NR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoter

Depositor

Lee Helman

Article

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

NR0B1 (NR0B1 Human)

Expression

Mammalian

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-Flag-H3.3

Plasmid

#47980

Purpose

tetracycline-inducible expression of human Flag tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Tags

Flag

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

CMV promoter with tetracycline operator

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pTMB304_ZFcharm_Kv1_Prnp_SCR

Plasmid

#220842

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199203

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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IGF2BP1_2xRRM-4xKH_pGEX

Plasmid

#135122

Purpose

Recombinant expression of IGF2BP1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

IGF2BP1 (IGF2BP1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMG023 MBP-Axin 384-518-His (miniAxin)

Plasmid

#154012

Purpose

Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

381-518 fragment

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSG5-hEKLF

Plasmid

#67835

Purpose

Expression of human EKLF driven by SV40 promoter for use in activation studies

Depositor

James Bieker

Article

Mas et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

EKLF (KLF1 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pHD-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199204

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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