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pCRISPR-HOT-Clover-BlastR

Plasmid

#138569

Purpose

Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

Depositor

Hans Clevers

Publication

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

Clover-BlastR

Use

CRISPR

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-Clover-BlastR

Plasmid

#138569

Purpose

Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

Depositor

Hans Clevers

Publication

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

Clover-BlastR

Use

CRISPR

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-GCaMP6s

Plasmid

#80146

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Publication

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Insert

GCaMP6s

Use

Lentiviral

Promoter

RSV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHAGE-RSV-GCaMP6s

Plasmid

#80146

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Publication

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Insert

GCaMP6s

Use

Lentiviral

Promoter

RSV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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MSGV Hu Acceptor

Plasmid

#64269

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

Depositor

David Ott

Publication

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

none

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSGV Hu Acceptor

Plasmid

#64269

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

Depositor

David Ott

Publication

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

none

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Publication

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Publication

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCM1.56

Plasmid

#17251

Depositor

Geraldine Seydoux

Publication

Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

Insert

L3787 promoter NO ATG in pDONRP4P1R

Use

Gateway Cloning

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pCM1.56

Plasmid

#17251

Depositor

Geraldine Seydoux

Publication

Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

Insert

L3787 promoter NO ATG in pDONRP4P1R

Use

Gateway Cloning

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-N1-Cdc42BPB

Plasmid

#50759

Purpose

expression of EGFP tagged Cdc42BPB

Depositor

Naoki Mochizuki

Publication

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

Cdc42BPB (CDC42BPB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-N1-Cdc42BPB

Plasmid

#50759

Purpose

expression of EGFP tagged Cdc42BPB

Depositor

Naoki Mochizuki

Publication

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

Cdc42BPB (CDC42BPB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmGFP-Sec16S

Plasmid

#15775

Depositor

Benjamin Glick

Publication

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16S (SEC16B Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N/A

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmGFP-Sec16S

Plasmid

#15775

Depositor

Benjamin Glick

Publication

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16S (SEC16B Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N/A

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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