We narrowed to 1,166 results for: ncl

1. PGL4.11-COL1A1double mutants

   Plasmid

   #230918

   Purpose

   Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase-luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   COL1A1 promoter with mutant SMAD and TEAD binding…

   Promoter

   COL1A1 with mutant SMAD and TEAD elements

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor smFP-FLAG (dark) WPRE

   Plasmid

   #131005

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-FLAG WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   FLAG - 10 total FLAG tags

   Expression

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nsp3 Mac1 - C2 (UCSF)

   Plasmid

   #169209

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Nsp3 Mac1 - P43 (Oxford)

   Plasmid

   #169210

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PB-EEA-5g-OSK2M2L1-PGK-Puro

   Plasmid

   #102909

   Purpose

   PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GG-EBNA-OMKSL-PP

   Plasmid

   #102899

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EcNR1 Strain

   Bacterial Strain

   #26930

   Depositor

   George Church

   Article

   Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

   Bacterial Resistance

   Ampicillin

   Available since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

8. DH10B E. coli aptI/gidB::Landing Pad

   Bacterial Strain

   #83036

   Purpose

   For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

   Depositor

   Cammie Lesser

   Article

   Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

   Bacterial Resistance

   Tetracycline

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

9. CC1215

   Bacterial Strain

   #79062

   Purpose

   BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.

   Depositor

   T. J. Kappock

   Bacterial Resistance

   Kanamycin

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Escherichia coli DH10B-MOB

    Bacterial Strain

    #229395

    Purpose

    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Bacterial Resistance

    60 ug/ml of dap

    Species

    Escherichia coli

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

11. Drosophila_sgRNA_group_3

    Pooled Library

    #134584

    Purpose

    Targets all other Drosophila genes not included in Groups 1 and 2

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Expression

    Insect

    Species

    Drosophila melanogaster

    Use

    CRISPR

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

12. Human ubiquitination-related proteins CRISPR KO library

    Pooled Library

    #174592

    Purpose

    CRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.

    Depositor

    Juan Bonifacino

    Article

    Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

13. Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library

    Pooled Library

    #138015

    Purpose

    This library can be used to investigate cardiac gene regulation. It targets transcriptional regulators that are expressed in mouse cardiomyocytes. A subset of GATA4 regulated genes are also included.

    Depositor

    William Pu

    Article

    VanDusen et al bioRxiv 2019, 808402v1; doi: https://doi

    Species

    Mus musculus

    Use

    AAV, CRISPR, and Cre/Lox

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

14. S. rosetta Col+ cDNA amplified library

    Pooled Library

    #62844

    Purpose

    amplified library of Salpingoeca rosetta from a colony rich culture which included the colony inducing bacteria Algoriphagus machipongonensis

    Depositor

    Nicole King

    Article

    Fairclough et al Genome Biol. 2013 Feb 18;14(2):R15. doi:

    Expression

    Bacterial

    Species

    Salpingoeca rosetta

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. EXPRESSYALI

    Plasmid Kit

    #1000000245

    Purpose

    The EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.

    Depositor

    Irina Borodina

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

16. AID* Kit

    Plasmid Kit

    #1000000120

    Purpose

    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

    Depositor

    Helle Ulrich

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

17. Yeast GPCR-sensor Toolkit

    Plasmid Kit

    #1000000157

    Purpose

    The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.

    Depositor

    Tom Ellis

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

18. C. elegans miniMos transgenesis kit

    Plasmid Kit

    #1000000031

    Purpose

    29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.

    Depositor

    Erik Jorgensen

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

19. MetClo DNA Assembly Kit

    Plasmid Kit

    #1000000153

    Purpose

    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.

    Depositor

    Christopher A. O'Callaghan

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

20. openCIDAR MoClo Toolkit

    Plasmid Kit

    #1000000227

    Purpose

    This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.

    Depositor

    Eric Young

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only