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pLC-Flag-CSNK1A1 WT-Puro

Plasmid

#123319

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-VAPA

Plasmid

#212096

Purpose

Fluorescent reporter for VAPA clearance to acidic lysosomes

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

VAPA (VAPA Human)

Use

Piggybac

Tags

mKeima

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe H-Ras 12V

Plasmid

#12545

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

H-Ras 12V (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated.

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-IKBalpha-wt

Plasmid

#15290

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgACSL4 clone2

Plasmid

#162129

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone1

Plasmid

#162128

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTNFR1-tdEOS

Plasmid

#98273

Purpose

mammalian expression of TNFR1-tdEOS

Depositor

Mike Heilemann

Article

Heidbreder et al Biochim Biophys Acta. 2012 Oct;1823(10):

Insert

TNFR1 (TNFRSF1A Human)

Tags

Flag and tdEos

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras 61L

Plasmid

#13485

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

HRas 61L (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

61L

Available Since

Dec. 20, 2006

Availability

Academic Institutions and Nonprofits only
Ai27

Plasmid

#34630

Purpose

Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

Floxed hChR2(H134R)-tdTomato

Use

Mouse Targeting

Tags

tdTomato

Mutation

His 134 has been mutated to Arg

Promoter

CAG

Available Since

Feb. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 G40N-Puro

Plasmid

#123320

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-hCD2-CD14 (4R)

Plasmid

#133062

Purpose

Retroviral expression of mouse CD14 mutant "4R" deficient in LPS binding

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

CD14 (4R) (Cd14 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

CD14 allele defective for LPS binding (mutations …

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone1

Plasmid

#162122

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLC-CUL4A K705R-P2A-Puro

Plasmid

#124305

Purpose

Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Lysine 705 to Arginine

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTH728-CEN-RLuc/maxCFLuc

Plasmid

#38212

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-dCas9-BirA*

Plasmid

#159994

Purpose

Bacterial expression of dCas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

dCas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTH726-CEN-RLuc/minCFLuc

Plasmid

#38210

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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FB009

Plasmid

#119707

Purpose

Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC::nptII::Ttub

Use

Synthetic Biology

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV10-mFoxP1-ES

Plasmid

#35169

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pLEX304-ARF5-mNeonGreen

Plasmid

#162025

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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