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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 50,540 results for: kan

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Showing: 11581 - 11600 of 50540 results

  1. pMax_BFP_A191G

    Plasmid
    #177823
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Publication
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tagBFP_A191G
    Expression
    Mammalian
    Mutation
    A191G
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177823-plasmid-map-sequence-id-348952

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  2. H2B-LSSmGFP

    Plasmid
    #186539
    Purpose
    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LSSmGFP
    Tags
    Human Histone 2B (D26G and V119I mutations)
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186539-plasmid-map-sequence-id-368219

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  4. pQ79

    Plasmid
    #175064
    Purpose
    expresses mutant of the huntingtin exon1 in mammalian cells
    Depositor
    Arnold Boersma
    Publication
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    polyQ79 (HTT Human)
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175064-plasmid-map-sequence-id-353796

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  5. pQ25-VC

    Plasmid
    #175062
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells
    Depositor
    Arnold Boersma
    Publication
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    polyQ25 (HTT Human)
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175062-plasmid-map-sequence-id-362344

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  6. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Publication
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Promoter
    T7lac promoter
    Available since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

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  10. R6K_tac_mCherry_ChInt

    Plasmid
    #187382
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherry
    Depositor
    Roi Avraham
    Insert
    mCherry
    Expression
    Bacterial
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    187382-plasmid-map-sequence-id-371124

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  12. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid
    #188072
    Purpose
    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.
    Depositor
    Kristen Verhey
    Publication
    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e
    Insert
    rat KIF5C (Kif5c Rat)
    Tags
    Flag and Halo
    Expression
    Mammalian
    Mutation
    aa1-560, deletion of aa2-6
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    188072-plasmid-map-sequence-id-368400

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  15. pNIC28-Bsa4_ZV-NS5-RdRp

    Plasmid
    #179847
    Purpose
    The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89
    Depositor
    Julien Lescar
    Publication
    Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00
    Insert
    Zika Virus Non-structural protein 5 RdRp domain
    Tags
    6-histine
    Expression
    Bacterial
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179847-plasmid-map-sequence-id-365329

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Showing: 11581 - 11600 of 50540 results