We narrowed to 22,641 results for: his

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Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Tags

6xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTE1059

Plasmid

#186628

Purpose

Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbA
scbB
scbC

Use

Synthetic Biology

Tags

6xHis and 6xHis-thrombin

Expression

Bacterial

Promoter

Synthetic promoter B10, Synthetic promoter B19, a…

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-E192C

Plasmid

#180233

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domains (residue…

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg88-PDGFR_EF1a_eBFP2

Plasmid

#186312

Purpose

Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg88-PDGFR
eBFP2

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-RBP-LOV2

Plasmid

#185792

Purpose

Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

Depositor

Lizhong He

Article

Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

Insert

RBP-LOV2

Tags

10xHis, TEV protease cleavage site after His Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_I770A_6xH

Plasmid

#185711

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed I770 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Tyr-P(11)4

Plasmid

#185784

Purpose

Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

Tyr-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-TmCA-P(11)4

Plasmid

#185783

Purpose

Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

TmCA-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-InPAkt

Plasmid

#181915

Purpose

Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

pm-InPAkt

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Contains the following mutations with respect to …

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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p130Cas Y15F

Plasmid

#186154

Purpose

For expressing recombinant p130Cas in insect cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6 (Tev)

Expression

Insect

Mutation

15Y residues mutated to Alanine

Promoter

polyhedrin

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Nephrin

Plasmid

#186134

Purpose

For expression of recombinant nephrin in bacteria cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NPHS1 (NPHS1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

Amino acid residues 1174-1223, with mutations: Y1…

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔCN

Plasmid

#183451

Purpose

Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

aa337-343 deleted

Promoter

CMV

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DrFLINC-AKAR-Kras

Plasmid

#182334

Purpose

Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

DrFLINC-AKAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dronpa-Kras

Plasmid

#182329

Purpose

Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Dronpa-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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WT (DVD)

Plasmid

#184316

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Article

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Tags

Hexahistidine followed by B-thymosin

Expression

Yeast

Promoter

AOX1

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pY71-PT7-RiboJ-sfGFP-MGapt

Plasmid

#129119

Purpose

Expresses a fluorescent reporter for the quantification of cell-free protein expression.

Depositor

Matthew Lux

Article

Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

Insert

Superfolder GFP with Malachite Green aptamer

Use

Synthetic Biology

Tags

His6 and RiboJ Insulator

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Kv1.2 K+ channel scFv [K14/16]

Plasmid

#182064

Purpose

Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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