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iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

Plasmid

#204727

Purpose

Frt-inducible CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pED1a6

Plasmid

#134787

Purpose

Luciferase reporter for quadruplet codon TAGA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-TAGA

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-TAGA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED1a20

Plasmid

#134788

Purpose

Luciferase reporter for quadruplet codon CAGG

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-CAGG

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-CAGG

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED1a34

Plasmid

#134789

Purpose

Luciferase reporter for quadruplet codon AGGG

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-AGGG

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-AGGG

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED1a36

Plasmid

#134790

Purpose

Luciferase reporter for quadruplet codon CGGT

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-CGGT

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-CGGT

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED1a39

Plasmid

#134791

Purpose

Luciferase reporter for quadruplet codon TACA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-TACA

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-TACA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED1a71

Plasmid

#134792

Purpose

Luciferase reporter for quadruplet codon AGGA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-AGGA

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-AGGA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED1a89

Plasmid

#134793

Purpose

Luciferase reporter for quadruplet codon CGGC

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-CGGC

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-CGGC

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAcrVIA4

Plasmid

#164863

Purpose

Inducible expression of AcrVIA4 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

AcrVIA4

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAcrVIA7

Plasmid

#164864

Purpose

Inducible expression of AcrVIA7 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

AcrVIA7

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGAT

Plasmid

#112587

Purpose

Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-GST

Expression

Bacterial

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTS36.6

Plasmid

#106264

Purpose

Expression of luc, ekivR, esyn1, mutated P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

luc-P2A*-ekivR-P2A*-esyn1

Use

Luciferase and Synthetic Biology

Mutation

P2A: changed final proline to alanine

Promoter

Tet-On

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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PP318

Plasmid

#78941

Purpose

Expresses GFP. Figure 3. Strain 5.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG292

Plasmid

#80586

Purpose

empty vector (lacZ) for 2nd generation recipient assembly

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG294

Plasmid

#80587

Purpose

dummy module (ccdB) for 2nd generation recipient assembly

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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PP297

Plasmid

#78937

Purpose

Expresses GFP. Figure 3. Strain 3.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP298

Plasmid

#78938

Purpose

Expresses GFP. Figure 3. Strain 4.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP299

Plasmid

#78939

Purpose

Expresses GFP. Figure 3. Strain 6.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP300

Plasmid

#78940

Purpose

Expresses GFP. Figure 3. Strain 7.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP296

Plasmid

#78936

Purpose

Expresses GFP. Figure 3. Strain 2.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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