168,033 results

1. pET-30-His6-SUMO-AEY-CfaN

   Plasmid

   #122949

   Purpose

   Bacterial expression of N-intein

   Depositor

   Thomas Muir

   Article

   Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

   Insert

   CfaN
   

   Expression

   Bacterial

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p3xFLAG CMV14 / BRAF-V600E

   Plasmid

   #131723

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   CMV

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15-UbE2W

   Plasmid

   #15809

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2W (UBE2W Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPAmCherry1-C1

   Plasmid

   #31929

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   CMV

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p5155

   Plasmid

   #175391

   Purpose

   Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-CD40L-mgSrtA

   Plasmid

   #125795

   Purpose

   for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.

   Depositor

   P. Chen

   Article

   Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

   Insert

   CD40L-mgSrtA
   

   Expression

   Mammalian

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFlagCMV2-UbcH8

   Plasmid

   #12442

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   UbcH8 (UBE2L6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

8. qTAG-N-Blast-ultraID

   Plasmid

   #207682

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. EcoFlex MoClo Kit

   Plasmid Kit

   #1000000080

   Purpose

   Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design

   Depositor

   Paul Freemont

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCAGGS-Akt-FoxO3a-KTR-EGFP

    Plasmid

    #125131

    Purpose

    Fluorescent reporter for Akt activity

    Depositor

    Kazuhiro Aoki

    Article

    Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

    Insert

    FOXO2 (FOXO3 Human)
    

    Tags

    Enhanced green fluorescent protein (EGFP)

    Expression

    Mammalian

    Mutation

    FoxO3A 1-402 amino acid

    Promoter

    CAG promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCRISPRi-v2, Stress and Proteostasis (m3), top 5 sgRNAs/gene

    Pooled Library

    #83991

    Purpose

    Mouse CRISPRi Pooled Library targeting stress and proteostasis genes

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3/Flag-WTAP

    Plasmid

    #53741

    Purpose

    For expression of WTAP (Wilms tumor 1 associated protein) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Wilms tumor 1 associated protein (WTAP Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1(+)-NEMOm

    Plasmid

    #189930

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOs
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SaCas9 GFP V3

    Plasmid

    #226962

    Purpose

    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1-CAG-hrGFP

    Plasmid

    #52344

    Purpose

    AAVS1 donor vector for constitutive gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    hrGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. zWnt5b

    Plasmid

    #21282

    Depositor

    Randall Moon

    Article

    Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

    Insert

    Wnt5b (wnt5b Zebrafish)
    

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

    Plasmid

    #98928

    Purpose

    Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1 and AAV5

    Insert

    Ruby2-smFP-Flag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMF440

    Plasmid

    #62550

    Purpose

    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

    Depositor

    Michael Franklin

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-INSR-V5/HIS

    Plasmid

    #201980

    Purpose

    expression of human INSR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSR receptor tyrosine kinase (INSR Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge