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pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA

Plasmid

#187066

Purpose

Gp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Gp41-1-ABEmaxNGA

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mhCMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PCTAIRE-1

Plasmid

#1996

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

PCTAIRE-1 (CDK16 Human)

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
Integrin alpha5(1-623)-AHCys

Plasmid

#27301

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

alpha 5(1-623)+GG+TEV+GGEN+AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-EPSIN 1

Plasmid

#22225

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Tags

Xpress

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTH760-CEN-mCherry_v3

Plasmid

#40598

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pWXLd-RIEP-OgNLuc

Plasmid

#135936

Purpose

Expresses the OgNLuc LumiFluor in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

Plasmid

#133342

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-Gal4DBD-CapN-SsrA-CapC

Plasmid

#213533

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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VV201: ChR2(C128S)-eGFP in fubi

Plasmid

#58510

Purpose

Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

ChR2(C128S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Cysteine 128 to Serine

Promoter

Ubiquitin

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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P74-26 Tail Tube Protein (gp93) in pSMT3

Plasmid

#191261

Purpose

Expresses P74-26 gp93 with a His-SUMO tag in bacterial cells

Depositor

Brian Kelch

Article

Agnello et al J Biol Chem. 2023 Feb 13:103021. doi: 10

Insert

P74-26 gp93

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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907H1

Plasmid

#160234

Purpose

FlightN-CymR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

p-cymene repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-Cyc

Plasmid

#33156

Depositor

Michael Rosbash

Article

Kadener et al PLoS Biol. 2008 May 20;6(5):e119.

Insert

Cyc (cyc Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pCAFNF-tdTomato

Plasmid

#125575

Purpose

Flp-dependent expression of tdTomato in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

tdTomato

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_W267N

Plasmid

#214311

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Mutation

W267N

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-PLAGL2

Plasmid

#133380

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSR33

Plasmid

#69254

Purpose

phsp16.2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

hsp16.2

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk3-DN-HA

Plasmid

#1880

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk3 (CDK3 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
miniSOG-Lifeact-7

Plasmid

#54823

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Lifeact-miniSOG

Tags

Lifeact

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits