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JAB2098

Plasmid

#190340

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-EFS:GFP

Plasmid

#68484

Purpose

Retrovirus expressing GFP driven by EFS promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

EFS and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4.11 promPDE4D3 Ebox mut

Plasmid

#27304

Depositor

David E. Fisher

Article

Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

Insert

prom PDE4D3 (PDE4D Human)

Use

Luciferase

Mutation

Ebox CACATG changed to GAGATG

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN12RRFuzzy3MYB

Plasmid

#194441

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, RR, Fuzzy3MYB

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPHAGE2-Myc-mTZAP

Plasmid

#87188

Purpose

lentiviral stable expression of Myc-TZAP (mouse) in mouse embryonic stem cells

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (Zbtb48 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

unknown

Available Since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Expression

Mammalian

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAL189

Plasmid

#22278

Purpose

Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pYC121

Plasmid

#63917

Purpose

Recipient overexpressing vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

MYC

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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p33Fn

Plasmid

#158155

Purpose

Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Mutation

Rice codon optimized

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBN312

Plasmid

#86734

Purpose

GFP with Frt sites for FLP-mediated knock-out of gene function in C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

GFP_Frt_KO

Expression

Worm

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pME-mKOFP2-CAAX

Plasmid

#75155

Purpose

Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTFP-NLS-blast

Plasmid

#136257

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTFP-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL-Stat5a1*6

Plasmid

#130669

Purpose

Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-LynEGFP

Plasmid

#133923

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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HS-DN-Plk1

Plasmid

#105949

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Plk1 (PLK1 Human)

Expression

Bacterial

Mutation

Dominant negative K82 R

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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hsa-miR-100 plix4.03

Plasmid

#121128

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-miR-100 (MIR100 Human)

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf3b

Plasmid

#158373

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3b

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MKRN1_H307E

Plasmid

#78756

Purpose

To overexpress MKRN1_H307E in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H307E

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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