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pcDNA-Flag-LmoCas6

Plasmid

#126485

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHO_pTEFmut7_CggR_ble

Plasmid

#124584

Purpose

Plasmid for genomic integration of CggR repressor regulated by the pTEFmut7 promoter

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

cggR (cggR Synthetic)

Use

Synthetic Biology

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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H2B-mGarnet

Plasmid

#104311

Purpose

codes for a fusion of fluorescent protein mGarnet and human histon 2B (H2B), makes the H2B visible in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

H2B-mGarnet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Pgr_beta-catenin-1

Plasmid

#107664

Purpose

RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

beta-catenin-1

Use

RNAi

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNR230

Plasmid

#79546

Purpose

BxbI attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-ICeuI-sHRPaNRX3beta

Plasmid

#79910

Purpose

piggyBac transposon vector, sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPaNRX3beta

Use

Piggybac

Expression

Mammalian

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)

Plasmid

#70745

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry-ssrA

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2(7Q)

Plasmid

#67679

Purpose

Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-mPlum-FRET-10

Plasmid

#58154

Purpose

Localization: Cytosolic FRET, Excitation: 504 / 590, Emission: 517 / 649

Depositor

Michael Davidson

Type

Empty backbone

Tags

mPA-GFP and mPlum

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pTorPE-GR-GECO1.1

Plasmid

#42198

Depositor

Robert Campbell

Article

Hoi et al J Am Chem Soc. 2012 Dec 26.

Insert

GR-GECO1.1

Tags

6His

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pCM3.06

Plasmid

#19602

Depositor

Geraldine Seydoux

Article

Lee et al PLoS Genet. 2007 Dec 28. 3(12):e233.

Insert

fbf-1 ORF+3' in pDONR201 (fbf-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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EFS-SpdCas9-Dnmt3A/3L-WT

Plasmid

#222497

Purpose

Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

Depositor

Isaac Hilton

Article

Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

Insert

S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

EFS

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

Plasmid

#197064

Purpose

Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Inserts

Encapsulin
Adaptor Protein

Use

CRISPR

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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G31, Cdc20-AID tagging

Plasmid

#130269

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Article

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)

Tags

AID(71-114)

Expression

Yeast

Promoter

Native promoter

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
AAV-EFS-GFP

Plasmid

#170176

Purpose

Expresses GFP under the control of EF1a short promoter

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

GFP

Use

AAV

Promoter

EFS promoter

Available Since

July 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-EGFP

Plasmid

#209057

Purpose

Gateway-compatible entry vector, with insert of the EGFP gene

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

EGFP

Use

Gateway Cloning

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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mini-donor - SOX17-EGFP

Plasmid

#206048

Purpose

this mini-donor knockin vector can be used to knock EGFP into SOX17 locus

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

nuclear EGFP

Use

Knockin donor vector

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

Plasmid

#194694

Purpose

CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

Plasmid

#194695

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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