We narrowed to 13,695 results for: crispr cas9

1. pYPQ265N2

   Plasmid

   #174001

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-UNG
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ265O2

   Plasmid

   #174002

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265L2

   Plasmid

   #174000

   Purpose

   Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

   Plasmid

   #211818

   Purpose

   AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   C-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. RFN_EGFP_81

   Plasmid

   #60073

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. RFN_EGFP_47

   Plasmid

   #60071

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLP857_α-syn:oScarlet KI donor

   Plasmid

   #239403

   Purpose

   AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

   Depositor

   Subhojit Roy

   Article

   Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

   Insert

   oScarlet
   

   Use

   AAV and CRISPR

   Tags

   oScarlet

   Expression

   Mammalian

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. RFN_EGFP_84

   Plasmid

   #60076

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. RFN_EGFP_83

   Plasmid

   #60075

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. RFN_EGFP_82

    Plasmid

    #60074

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. RFN_EGFP_80

    Plasmid

    #60072

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLY54

    Plasmid

    #130923

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLY9

    Plasmid

    #130907

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY59

    Plasmid

    #130927

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and MCP (MS2 coat protein)

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY55

    Plasmid

    #130924

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLY53

    Plasmid

    #130911

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM6-G6-vioABE-4A6-vioC

    Plasmid

    #73439

    Purpose

    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    PG6 and P4A6 (orthogonal T7-lac variants)

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJOG252

    Plasmid

    #80584

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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