We narrowed to 26,146 results for: Nes;

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

Plasmid

#69906

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLVTH-sip53

Plasmid

#12239

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against p53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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RCAN1

Plasmid

#65413

Purpose

Full length RCAN1 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1 (Rcan1 Mouse)

Tags

Flag tagged

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mVenus

Plasmid

#154899

Purpose

Expresses a fluorescent protein, mVenus

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

mVenus

Expression

Mammalian

Promoter

EF-1α

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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3279 pSFFV-neo Bid human

Plasmid

#8766

Depositor

Stanley Korsmeyer

Article

Wang et al Genes Dev 1996 Nov 15;10(22):2859-69.

Insert

Bid ORF cDNA FL (BID Human)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_nHA Spinophilin

Plasmid

#87122

Purpose

mammalian expression of Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242/287A

Plasmid

#32822

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242/287A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
SB52

Plasmid

#21487

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174529

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Inserts

PylS
PylT

Tags

Flag

Expression

Mammalian

Promoter

U6

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AIRAP 9: PSMD2.GST.V2.pcDNA3

Plasmid

#21799

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD2 (PSMD2 Human)

Tags

GST

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F2

Plasmid

#184167

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F1

Plasmid

#184166

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113343

Purpose

Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

p300core-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_BAP_his

Plasmid

#210402

Purpose

For expression of VHH with C-terminal biotin acceptor peptide and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

biotin acceptor peptide-his6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pETCON-PTX-S1-220

Plasmid

#67747

Purpose

Pertussis Toxin Subunit 1 Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

PTxS1-220

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-CICf

Plasmid

#48185

Purpose

Expresses Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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