We narrowed to 3,058 results for: BFP

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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-BFP

Plasmid

#188776

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

Plasmid

#205161

Purpose

express β-ACTIN-3′UTR-24xMS2

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

β-ACTIN-3′UTR-24xMS2

Tags

tagBFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186A-T2A-BFP

Plasmid

#194932

Purpose

LV construct encoding Y186A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y192A-T2A-BFP

Plasmid

#194933

Purpose

LV construct encoding Y192A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-sNRR-T2A-BFP

Plasmid

#194930

Purpose

LV construct encoding sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-R103A-T2A-BFP

Plasmid

#194935

Purpose

LV construct encoding R103A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

Plasmid

#173029

Purpose

For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

Depositor

Mohamed Fareh

Article

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

pspCas13b

Use

CRISPR and Lentiviral

Tags

3xFLAG, BFP, HIV NES, and T2A

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

Plasmid

#64216

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-BLM-UGI-T2A-mTagBFP2

Plasmid

#221222

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-BLM-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP

Plasmid

#64323

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-EBFP2

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

Plasmid

#242407

Purpose

Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-moxBFP

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

Plasmid

#225754

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

Plasmid

#200631

Purpose

Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

iCasp9

Use

Lentiviral

Promoter

pTRE3G

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-NRR-T2A-BFP

Plasmid

#194929

Purpose

LV construct encoding NRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-NRR-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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