We narrowed to 1,604 results for: N-pac

1. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

   Plasmid

   #228251

   Purpose

   pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
   

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-PB2_Lock1-I436C+S993C

   Plasmid

   #182879

   Purpose

   Lentivirus for expression of Plexin-B2 locked ring mutant

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

   Insert

   hPLXNB2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Mutation

   changed Isoleucine 436 to Cysteine and Serine 993…

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-PB2_Lock2-I436C+T1051C

   Plasmid

   #182880

   Purpose

   Lentivirus for expression of Plexin-B2 locked ring mutant

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

   Insert

   hPLXNB2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Mutation

   changed Isoleucine 436 to Cysteine and Threonine …

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-RPS14(89-151)

   Plasmid

   #122299

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1 to 88

   Promoter

   CMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-RPS14(2-88)

   Plasmid

   #122298

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (partial) (RPS14 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 89 to 151

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAD-IRP

   Plasmid

   #220751

   Purpose

   Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Insert

   Iron Regulatory Protein (ACO1 )
   

   Expression

   Yeast

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2ADWpuro_2paRAF

   Plasmid

   #129653

   Purpose

   For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2ADW_2paRAF

   Plasmid

   #129654

   Purpose

   For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

   Plasmid

   #164266

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6 promoter from W05B2.8

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcry:GFP,-503unc:hACTA1GFP

    Plasmid

    #172529

    Purpose

    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.

    Depositor

    Joachim Berger

    Article

    Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

    Insert

    ATCA1
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcry:mCherry,-600unc:cct3GFP

    Plasmid

    #172528

    Purpose

    alphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.

    Depositor

    Joachim Berger

    Article

    Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

    Insert

    cct3-GFP
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-codon optimized catalytically inactive PHGDH

    Plasmid

    #154903

    Purpose

    Expresses codon optimized catalytically inactive PHGDH in mammalian cells

    Depositor

    Michael Pacold

    Article

    Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Three mutations: D175N, R236K, H283A

    Promoter

    TRE

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-miniTurbo-V5-SCYL3_IDG-K

    Plasmid

    #170606

    Purpose

    Gateway destination clone of SCYL3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-SCYL3 (SCYL3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Adeno Cas9

    Plasmid

    #64072

    Purpose

    Adenoviral vector for the expression of the Cas9

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    FLAG

    Promoter

    U6 and CBh

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. tetO-TBX5

    Plasmid

    #46032

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKD282

    Plasmid

    #125072

    Purpose

    Expression of SO_4427 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4427 (SO_4427 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR305

    Plasmid

    #124712

    Purpose

    Expression of uhpB under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    uhpB (uhpB )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKD288

    Plasmid

    #125075

    Purpose

    Expression of SO_4638 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4638 (SO_4638 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    mCherry*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138734

    Purpose

    Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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