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Showing: 1161 - 1180 of 31524 results

  1. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

    Plasmid
    #162381
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    A25G F286Y
    Promoter
    CAG
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    162381-plasmid-map-sequence-id-413019

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  6. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Publication
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles

  9. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid
    #174007
    Purpose
    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.
    Depositor
    Mark Histed
    Publication
    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22
    Has service
    AAV9
    Insert
    jGCaMP8s-P2A-ChrimsonR-ST
    Use
    AAV
    Tags
    6xHis and soma targeting motif
    Promoter
    hSyn
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    174007-plasmid-map-sequence-id-361587

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  11. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Publication
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    69835-plasmid-map-sequence-id-183106

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  18. pAAV-CAG-DIO-ChR2(H134R)-eYFP

    Plasmid
    #127090
    Purpose
    Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation
    Depositor
    Viviana Gradinaru
    Publication
    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:
    Has service
    AAV PHP.eB
    Insert
    Channelrhodopsin-2
    Use
    AAV
    Tags
    eYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    Humanized ChR2 gene with histidine 134 changed to…
    Promoter
    CAG
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    127090-plasmid-map-sequence-id-246739

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  20. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Publication
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    158757-plasmid-map-sequence-id-310662

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Showing: 1161 - 1180 of 31524 results