We narrowed to 17,421 results for: tes
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Plasmid#61412PurposeExpresses human OTUD3 (OTU domain) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 210-398.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-2xFYVE_hrs
Plasmid#67676PurposeMultisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrsDepositorInsert2xFYVEhrs
UseGateway multisite 3' entry cloneAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K6R
Plasmid#121153PurposeMammalian expression of HA tagged ubiquitin K6RDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHDM-VSV-G
Plasmid#164440PurposeEncodes VSV-G protein for pseudovirus productionDepositorInsertVSV-G
ExpressionMammalianAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-FR-GECO1c
Plasmid#163682PurposeFar-red fluorescent calcium indicator FR-GECO1cDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFR-GECO1c
ExpressionMammalianAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
ExpressionBacterialAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G protein alpha-q-GFP
Plasmid#66080PurposeThis G protein alpha-q construct contains internal insertions of GFP and the EE epitopeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG protein alpha-q-EE-YFP (Gnaq Mouse)
TagsGFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-NES-NIR-GECO1
Plasmid#113683PurposeExpress NIR-GECO1 preferentially in neuronsDepositorInsertNES-NIR-GECO1
UseAAVPromoterhSynAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Trak1
Plasmid#127621PurposeN-terminal GFP-tagged mouse Trak1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrak1 (Trak1 Mouse)
TagseGFPExpressionMammalianAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BNLS
Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddGFP B
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_Zeo
Plasmid#118596PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
ExpressionMammalianPromoterCMVAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CDK1as_T2A_puromycin
Plasmid#118595PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
ExpressionMammalianPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-mKate2
Plasmid#67671PurposeMultisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2DepositorInsertAPEX2-P2A-mKate2
UseGateway multisite 3' entry cloneAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
NESRA-DEVD-BNLS
Plasmid#50849PurposeExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A and ddRFP B
TagsNES sequence LQKKLEELELDE placed after ddGFP A an…ExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
ML1Nmut_in_pEGFP-C1
Plasmid#67796PurposeMammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertML1N*2(7Q)-EGFP
ExpressionMammalianPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKate2-P2A-APEX2-TAPP1-PH
Plasmid#67662PurposeMammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmKate2-P2A-APEX2-TAPP1-PH
ExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-pmExRai-AKAR2
Plasmid#161755PurposePlasma membrane-targeted ExRai-AKAR2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn-ExRai-AKAR2
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-FR-GECO1a
Plasmid#163681PurposeFar-red fluorescent calcium indicator FR-GECO1aDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFR-GECO1a
ExpressionMammalianAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddGFP A and ddGFP B
TagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by