We narrowed to 2,663 results for: ubiquitin

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FLAG-OTUB1 T134R,C91S-pcDNA3.1

Plasmid

#118212

Purpose

Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine and threonine 134 t…

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LZRS ATG12 ires zeo

Plasmid

#67531

Purpose

For retroviral based stable expression of human ATG12

Depositor

Stephen Tait

Article

Haller et al Autophagy. 2014;10(12):2269-78. doi: 10.

Insert

APG12 (ATG12 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (925-953)

Plasmid

#73744

Purpose

Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL WT

Plasmid

#27040

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 EPSM A204S

Plasmid

#27111

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V S204A S208A S213A; A204S

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A204S

Plasmid

#27108

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A204S

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (918-932)

Plasmid

#73748

Purpose

Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-932 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM V179T

Plasmid

#27015

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; V179T

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A208S

Plasmid

#27109

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A208S

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A213S

Plasmid

#27110

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A213S

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC AAGY

Plasmid

#27039

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; P181A P182A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 AAGY

Plasmid

#27004

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

P181A P182A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC WT

Plasmid

#26996

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPc1 Rab34

Plasmid

#237338

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

Rab34 (RAB34 Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-HOIL-1L NZF (195-252)

Plasmid

#235650

Purpose

Bacterial expression of human HOIL-1L NZF (195-252)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

HOIL-1L NZF (RBCK1 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 195-252 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 (N)ZF1-3 (1-173)

Plasmid

#235646

Purpose

Bacterial expression of human CAPN15 (N)ZF1-3 (1-173)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 (N)ZF1-3 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-173 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10

Plasmid

#226351

Purpose

Expression of Titin (V15P) substrate for 26S proteasome

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

titin (TTN Human)

Expression

Bacterial

Mutation

V15P

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-RIPLET ΔPRY/SPRY

Plasmid

#218516

Purpose

RIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1

Depositor

Sun Hur

Article

Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

Insert

RIPLET delta PRY/SPRY domain (RNF135 Human)

Expression

Mammalian

Available Since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A

Plasmid

#175752

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Lentiviral

Mutation

Y49A L50A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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