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SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFP

Plasmid

#244208

Purpose

Mammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

Plasmid

#244213

Purpose

Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

Plasmid

#244216

Purpose

Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM280_EF1A-dEnAsCas12a-4xSID-T2A-BlastR

Plasmid

#244215

Purpose

Mammalian expression of dEnAsCas12a-4xSID with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM279_EF1A-dEnAsCas12a-4xSID-T2A-EGFP

Plasmid

#244214

Purpose

Mammalian expression of dEnAsCas12a-4xSID with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_Neg_pBADProm

Plasmid

#242141

Purpose

pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III Negative

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_Pos_pBADProm

Plasmid

#242139

Purpose

pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_GIII_Titrate

Plasmid

#242140

Purpose

PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_SP_SD4_AraC

Plasmid

#242143

Purpose

Contains an AraC gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_sd2_R2_C1

Plasmid

#242149

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2

Expression

Bacterial

Mutation

E3K, R146L, A152T, R251L

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_sd2_R5_C1

Plasmid

#242150

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R5

Expression

Bacterial

Mutation

A2E, L156I, R251H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-TCGC-mNeongreen

Plasmid

#241203

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-mCherry-pA

Plasmid

#241212

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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BsaI-MCS-cloning vector for golden gate

Plasmid

#241214

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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SA-memb-mNeongreen

Plasmid

#241207

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-dest-BsaI-cmlc2:mCherry

Plasmid

#241213

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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SA-EGFP-pA

Plasmid

#241211

Purpose

Middle entry vector for mini-Golden subcloning platform; eGFP

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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memb-mNeongreen

Plasmid

#241175

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA

Plasmid

#200542

Purpose

mini circle middle entry plasmid for CreER-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_mCherry-pA

Plasmid

#200521

Purpose

Mini circle middle entry plasmid for mCherry- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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