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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai3

Plasmid

#16370

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai3 (ORAI3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pRS424GFP-2xPH(Osh2)

Plasmid

#36095

Depositor

Scott Emr

Article

Stefan et al Cell. 2011 Feb 4;144(3):389-401.

Insert

2xPH(Osh2) fusion

Tags

GFP

Expression

Yeast

Promoter

PRC1

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
Butelase AY

Plasmid

#221847

Purpose

Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

Val237Ala, Thr238Tyr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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minCMV-1xmNG-dMCP

Plasmid

#231091

Purpose

Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-ssRFP-GFP-KDEL

Plasmid

#128257

Purpose

Fluorescence reporter to monitor ER-phagy activity

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

ssRFPGFPKDEL

Use

Lentiviral

Tags

RFPGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Sumire-pRsetB

Plasmid

#193361

Purpose

GFP variant emit 414 nm fluorescence.

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

Sumire

Use

E.coli expression

Tags

His X6 tag

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGL0_142 [EilR-PjexD]

Plasmid

#199026

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

EilR-PjexD

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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minCMV-1xmNG-dPCP

Plasmid

#231092

Purpose

Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLSLR

Plasmid

#51500

Purpose

Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Plant t-dna plasmid

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGBSedit Sham

Plasmid

#223200

Purpose

Group B Streptococcus Efficient Genome Editing with Modular crRNA

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_sfCherry2(1-10)

Plasmid

#82602

Purpose

Expresses sfCherry2(1-10) fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(1-10)

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc S36A (corrected)

Plasmid

#32575

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Mutation

S36A

Promoter

CMV

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-tTA

Plasmid

#104109

Purpose

AAV-mediated expression of tTA under the Syn1 promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Use

AAV

Promoter

hSyn1

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNF02-mChartreuse

Plasmid

#219397

Purpose

Constitutive expression of mChartreuse in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mChartreuse

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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