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pMSCV-IRES-GFP-human-Caspase-4

Plasmid

#183356

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-GPAT4152-208

Plasmid

#134468

Purpose

GPAT4152-208 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

GPAT4 (GPAT4 Human)

Expression

Mammalian

Mutation

GPAT4, amino acids 152-208

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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spCas9-NLS-MAV

Plasmid

#124212

Purpose

SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

Depositor

Uri David Akavia

Article

Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

Insert

cas9

Use

CRISPR

Tags

His6, MBP, TEV Cleavage site, and two copies of S…

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

Plasmid

#112004

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6s-P2A-mKate2

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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Cerulean-2xFv-hRIPK3

Plasmid

#106079

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

Cerulean-2xFv-hRIPK3 (RIPK3 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only
G10 Cas9 MDC123

Plasmid

#59187

Purpose

G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

G10

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Article

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-WT

Plasmid

#78920

Purpose

Mammalian expression of RNF138 with an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pGex-4T-2_GATE-16

Plasmid

#73518

Purpose

For recombinant expression of human GATE-16/GABARAPL2 in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GATE-16 (GABARAPL2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-flex-TVA

Plasmid

#69618

Purpose

Express recepter TVA for envA with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)

Use

AAV and Cre/Lox

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Short

Plasmid

#47394

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

Intersectin short (ITSN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pRK-HA-TRAF5

Plasmid

#58315

Purpose

HA-tagged mouse TRAF5

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 5 (Traf5 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-FlagmuEzh2deltaset-Hygro

Plasmid

#49403

Purpose

Expresses a Delta-set deletion mutant of Flag Tagged Ezh2. Hygromycin resistant.

Depositor

Tobias Neff

Insert

3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Set domain deleted

Promoter

MSCV promotor

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-FNIP1

Plasmid

#49175

Purpose

Mammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin Interacting Protein 1 (Fnip1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 2

Plasmid

#36102

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 2 (GNG2 Bovine)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pHA-ΔN331-Kin1B

Plasmid

#31603

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN331 kinesin-1B (Kif5b Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-331

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
mCol.2lox4F2A targeting construct

Plasmid

#25795

Depositor

Rudolf Jaenisch

Article

Carey et al Nat Methods. 2010 Jan . 7(1):56-9.

Insert

loxP-Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc-loxP

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-PTB

Plasmid

#21929

Depositor

Phil Sharp

Article

Garcia-Blanco et al Genes Dev. 1989 Dec . 3(12A):1874-86.

Insert

PTB (PTBP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag A)

Plasmid

#16605

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag A) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragA (XmnI->KpnI). The KpnI site is at -1194 …

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
Ngn3 Cre-ER (DM#256)

Plasmid

#15024

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Ngn3 promoter (Neurog3 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre-ER(TM)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only