Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,357 results for: BASE

Clear keywords
Showing: 11861 - 11880 of 13357 results

  12. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

    Enlarge

  13. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid
    #223123
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9=D10A
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223123-plasmid-map-sequence-id-447704

    Enlarge

  14. EcNGRfC

    Bacterial Strain
    #48936
    Purpose
    Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Depositor
    George Church
    Bacterial Resistance
    Spectinomycin
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. KJ740(DE3)

    Bacterial Strain
    #179486
    Purpose
    KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Bacterial Resistance
    Tetracycline (chromosome, Tn10) (10 μg/mL); Streptomycin/Spectinomycin (chromosome) (50 μg/mL)
    Species
    Escherichia coli
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only

  17. pHN872

    Plasmid
    #178830
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A
    Tags
    GST, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178830-plasmid-map-sequence-id-351931

    Enlarge

  18. pFastBacMam4_N_Flag_His8_eGFP_TEV

    Plasmid
    #178808
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    Flag, His8, eGFP, TEV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178808-plasmid-map-sequence-id-351925

    Enlarge

Showing: 11861 - 11880 of 13357 results