We narrowed to 14,311 results for: SHI

Showing: 11921 - 11940 of 14311 results

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psiCHECK-1-BRAF_Mut1-CM

Plasmid

#215922

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut1 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut2-CM

Plasmid

#215923

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-mmDNMT1

Plasmid

#205635

Purpose

reRNA guide targeting Mus Musculus DNMT1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmDNMT1 (Dnmt1 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-scFVDnmt3A_bGHpA

Plasmid

#177349

Purpose

AAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

598–908 aa of mouse Dnmt3a

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-scFVDnmt3L_bGHpA

Plasmid

#177356

Purpose

AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

194–415 aa

Promoter

CMV promoter

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en1dFnCas9GFP

Plasmid

#201950

Purpose

Expression of en1dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en1dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1369R on FnCas9

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en15dFnCas9GFP

Plasmid

#201951

Purpose

Expression of en15dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en15dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1603H on FnCas9

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dSpCas9

Plasmid

#201953

Purpose

Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dSpCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A on SpCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-113-117-5A

Plasmid

#213643

Purpose

Expresses human SKP1-113-117-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-113-117-5A (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-138-142-5A

Plasmid

#213645

Purpose

Expresses human SKP1-138-142-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-138-142-5A (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-120-124-5A

Plasmid

#213644

Purpose

Expresses human SKP1-120-124-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-120-124-5A (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-RR (K142R/K163R)

Plasmid

#213425

Purpose

Expresses human SKP1 K142R/K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R/K163R (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-F139A/I141A

Plasmid

#213687

Purpose

Expresses human SKP1-F139A/I141A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-F139A/I141A (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

Plasmid

#207493

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-851_HA-GD2-28z_HA-TFAP4

Plasmid

#207501

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NEMF_CDKL1

Plasmid

#205867

Purpose

Express mEGFP-tagged fusion protein, NEMF_CDKL1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NEMF_CDKL1 (NEMF, CDKL1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CTNNB1_ULK4

Plasmid

#205799

Purpose

Express mEGFP-tagged fusion protein, CTNNB1_ULK4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CTNNB1_ULK4 (CTNNB1, ULK4 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_AFF1_RAD51B

Plasmid

#205769

Purpose

Express mEGFP-tagged fusion protein, AFF1_RAD51B from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

AFF1_RAD51B (AFF1, RAD51B Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EP400_SFSWAP

Plasmid

#205810

Purpose

Express mEGFP-tagged fusion protein, EP400_SFSWAP from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EP400_SFSWAP (EP400, SFSWAP Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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