168,238 results

1. pSBbi-BB

   Plasmid

   #60521

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdTomato-anti-AlfaTag nanobody

   Plasmid

   #171819

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   tdTomato-anti-AlfaTag nanobody
   

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CBh-dCas9-Mll3SET

   Plasmid

   #108452

   Purpose

   Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter

   Depositor

   Bing Ren

   Article

   Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:

   Insert

   dCas9, MLL3 (Kmt2c Mouse)
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b Hot-PETase

   Plasmid

   #225761

   Purpose

   Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   Hot-PETase
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-3XHA-p53

   Plasmid

   #196267

   Purpose

   Mammalian expression of 3xHA tagged wild-type p53

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   p53 (Trp53 Mouse)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST exorh:mCherry

   Plasmid

   #195989

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann, Nicolas Rohner

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-EGFP-ALDH1A1-Neo

   Plasmid

   #189742

   Purpose

   The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A1 (ALDH1A1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_human CDK1

   Plasmid

   #118597

   Purpose

   One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   guide RNA against human CDK1
   

   Use

   CRISPR

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-HisD-miRFP720-HO1

   Plasmid

   #197257

   Purpose

   Expresses the protein of miRFP720 and HO1 in bacteria

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP720
   

   Expression

   Bacterial

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3HA-ERHBD-Hoxb8

    Plasmid

    #212932

    Purpose

    Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

    Depositor

    Hans Haecker

    Article

    Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

    Insert

    3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)
    

    Use

    Retroviral

    Tags

    Triple HA tag

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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