We narrowed to 300 results for: nisch

1. pMF-TetTight

   Plasmid

   #193288

   Purpose

   Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

   Depositor

   Rudolf Jaenisch

   Article

   Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

   Insert

   rtTA-P2A-tTS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF‐1α

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fuw-dCas9-Tet1CD

   Plasmid

   #84475

   Purpose

   Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fuw-dCas9-Dnmt3a_IM

   Plasmid

   #84478

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a_IM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGKpuro

   Plasmid

   #11349

   Depositor

   Rudolf Jaenisch

   Article

   Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fuw-dCas9-Tet1CD_IM

   Plasmid

   #84479

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD_IM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC150-PBLHL-4xHS-EF1a-DEST

   Plasmid

   #48234

   Purpose

   PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. INS-2A-luciferase-2A-tdT

   Plasmid

   #159348

   Purpose

   Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Insert

   Luciferase-2A-tdTomato
   

   Use

   Ins locus knockin donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TetO-FUW-oct4

   Plasmid

   #20323

   Depositor

   Rudolf Jaenisch

   Article

   Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

   Insert

   oct4 (Pou5f1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetO-Nr5a1

    Plasmid

    #41081

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Nr5a1 (Nr5a1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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