We narrowed to 4,220 results for: Pet2

1. pET29b-PD

   Plasmid

   #110168

   Purpose

   Expresses propanediol dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Propanediol Dehydratase
   

   Use

   Tags

   His6 and S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b_PfSSB_W166C

   Plasmid

   #103001

   Purpose

   The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteine

   Depositor

   Martin Webb

   Article

   Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

   Insert

   PfSSB_W166C
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C93A, W166C

   Promoter

   T7

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a_Msm_RbpA

   Plasmid

   #87928

   Purpose

   Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

   Depositor

   Seth Darst

   Article

   Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

   Insert

   RbpA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7P

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET24a_UDP_Xase_E162

   Plasmid

   #73160

   Purpose

   Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

   Depositor

   Sandra Gabelli

   Article

   Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

   Insert

   pET24a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   change glutamate 162 to alanine

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pET24_BD3179

   Plasmid

   #73142

   Purpose

   Expresses in E.coli B. bacteriovorus Bd3179

   Depositor

   Sandra Gabelli

   Article

   de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

   Insert

   Bd3179
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pet28a_RBPMS_1_120

   Plasmid

   #65737

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 1-120

   Promoter

   T7

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pet28a_RBPMS_1_144

   Plasmid

   #65739

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 1-144

   Promoter

   T7

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pet23a_RBPMS_20_196

   Plasmid

   #65734

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 20-196

   Promoter

   T7

   Available since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pet23a_RBPMS_1_129

   Plasmid

   #65731

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 1-129

   Promoter

   T7

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pet23a_RBPMS_1_111

    Plasmid

    #65729

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-111

    Promoter

    T7

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pet28a_RBPMS_20_196

    Plasmid

    #65741

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 20-196

    Promoter

    T7

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PA1421_WT_pET21a

    Plasmid

    #29792

    Depositor

    SeWon Suh

    Insert

    Guanidinobutyrase (gbuA P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. PA0288_WT_pET21a

    Plasmid

    #29793

    Depositor

    SeWon Suh

    Insert

    3-guanidinopropionase (gpuA P. aeruginosa)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2012

    Availability

    Academic Institutions and Nonprofits only

14. PA1421_M161Y_pET21a

    Plasmid

    #29791

    Depositor

    SeWon Suh

    Insert

    Guanidinobutyrase (gbuA P. aeruginosa)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M161Y

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. PA0288_Y157M_pET21a

    Plasmid

    #29794

    Depositor

    SeWon Suh

    Insert

    3-guanidinopropionase (gpuA P. aeruginosa)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y157M

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

16. PA RdgC_pET21a

    Plasmid

    #14927

    Depositor

    SeWon Suh

    Article

    Ha et al Nucleic Acids Res. 2007 Apr 10. ():.

    Insert

    Recombination-associated protein rdgC
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET21a.MJ1228

    Plasmid

    #11410

    Depositor

    Sung-Hou Kim

    Article

    Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

    Insert

    eukaryotic translation initiation factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pET21a.AQ_1575

    Plasmid

    #11416

    Depositor

    Sung-Hou Kim

    Article

    Shin et al Proc Natl Acad Sci U S A. 2002 Jun 11. 9

    Insert

    hypothetical (aq_1575 A. aeolicus)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pET28aAviHisCD72aCTLD

    Plasmid

    #129065

    Purpose

    generation of biotinylated mouse CD72a CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Article

    Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

    Insert

    CD72a (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28aHisAviCD72aCTLD

    Plasmid

    #129067

    Purpose

    generation of biotinylated mouse CD72a CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    CD72a (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28aHisAviCD72cCTLD

    Plasmid

    #129068

    Purpose

    generation of biotinylated mouse CD72c CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    CD72c (Cd72 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28aHisAvihCD72CTLD

    Plasmid

    #129069

    Purpose

    generation of biotinylated human CD72 CTLD protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    CD72 (CD72 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24_NusA-Tus

    Plasmid

    #165959

    Purpose

    Expression of soluble E. coli Tus protein

    Depositor

    Keith Pardee

    Article

    Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

    Insert

    Tus
    

    Use

    Tags

    His-NusA-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-PKM2-WT

    Plasmid

    #122690

    Purpose

    Express His-tagged PKM2 (wild type) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-PTBP1CCtoSS

    Plasmid

    #89157

    Purpose

    Recombinant expression of PTBP1 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NylCA-TS_pET28a

    Plasmid

    #215419

    Purpose

    Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCA-TS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S111G/A137L/E263Q

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. UMG-SP-3_pET29b

    Plasmid

    #215435

    Purpose

    Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ube1/PET21d

    Plasmid

    #34965

    Depositor

    Cynthia Wolberger

    Article

    Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

    Insert

    Ube1 (UBA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Use

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LCC-ICCG_pET21b

    Plasmid

    #215408

    Purpose

    Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    LCC-ICCG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F243I/D238C/S283C/Y127G

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-MSP1D1delltaH5

    Plasmid

    #71714

    Purpose

    Membrane scaffold protein (MSP), helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET23 P53DBD

    Plasmid

    #174503

    Purpose

    express P53 DNA binding domain (94-312) in E.coli without tag

    Depositor

    Stewart Loh

    Article

    Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

    Insert

    P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. tau/pET29b

    Plasmid

    #16316

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    tau (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge