Addgene

We narrowed to 407 results for: RPA

Showing: 201 - 240 of 407 results

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pCI-neo-HA-hApg12

Plasmid

#22950

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

autophagy related genes 12 (ATG12 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCI-neo-Myc-hApg12

Plasmid

#22951

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

autophagy related genes 12 (ATG12 Human)

Use

Tags

3xMyc

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
A1-LCD -3D+3N

Plasmid

#234614

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DN (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD -4D+4N

Plasmid

#234611

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DN (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N, D262N

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD -4D+4V

Plasmid

#234610

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DV (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V, D262V

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD -3D+3V

Plasmid

#234613

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DV (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_RtoK

Plasmid

#139120

Purpose

express hnRNPA2 LC where all R are K

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

all R to K

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_NtoS

Plasmid

#139121

Purpose

express hnRNPA2 with all N changed to S

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

all N to S

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_5E

Plasmid

#139122

Purpose

express hnRNPA2 with 5 serine changed to glutamate

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

5 serine to glutamate

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_12E

Plasmid

#139123

Purpose

express hnRNPA2 with all serine change to glutamate

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

12 S to E

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPF_PLD_YtoS

Plasmid

#139124

Purpose

express hnRNPF PLD with all Y changed to S

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

all Y to S

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPF_PLD_StoA

Plasmid

#139125

Purpose

express hnRNPF PLD with all S changed to A

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

all S to A

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-HNRNPA1C-mCh-Cry2WT

Plasmid

#101226

Purpose

hnRNPA1(186-320) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

hnRNPA1(186-320) (HNRNPA1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_RRMs

Plasmid

#157864

Purpose

express His tagged hnRNPA2 RRMs

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

190stop

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hRAP-pEZT-BM

Plasmid

#177986

Purpose

Expression human RAP containing a C-terminal 6xHis tag in mammalian cells

Depositor

Lora Hooper

Article

Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

Insert

LDL receptor related protein associated protein 1 (LRPAP1 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_RRM2

Plasmid

#157866

Purpose

express His tagged hnRNPA2 RRM2

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_RRM1

Plasmid

#157865

Purpose

express His tagged hnRNPA2 RRM1

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_nocharge

Plasmid

#139118

Purpose

express hnRNPA2 LC with no charged residues

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

all charged residues are changed to S/Q except fo…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-hApg5(K130R)-HA

Plasmid

#22949

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

130 Lysine to Arginine

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pJ4M_hnRNPA2_FL_D290V

Plasmid

#139110

Purpose

Express FL hnRNPA2 D290V with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

D290V

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_266-341

Plasmid

#118821

Purpose

Bacterial expression of hnRNPA2 truncation

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

contains amino acids 266-341

Promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
S

Plasmid

#167172

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S
ATP1B1

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ4M_hnRNPA2_FL_P298L

Plasmid

#139111

Purpose

Express FL hnRNPA2 P298L with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

P298L

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD D262V

Plasmid

#234607

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262V (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD D262N

Plasmid

#234608

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_D262N (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD -3G1S+4V

Plasmid

#234612

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to Asn

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4GSV (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

G204V, S231V, G274V, G303V

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD 5W D262V

Plasmid

#234617

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_5W D262V (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

F222W, F228W, Y237W, D262V, Y305W, F320W

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1347

Plasmid

#142378

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RPA3 (RPA3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_nocharge_wR

Plasmid

#139119

Purpose

express hnRNPA2 LC with no charged residues with R added back

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

remove all K, most D

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-hnRNPA2_FL_WT

Plasmid

#98662

Purpose

Expresses MBP-tagged full length hnRNPA2

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
WT A1-LCD +NLS

Plasmid

#178658

Purpose

Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

WT A1-LCD +NLS (HNRNPA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_WT

Plasmid

#98657

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-hnRNPA2_LC

Plasmid

#98661

Purpose

Expresses MBP-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_190-303

Plasmid

#118822

Purpose

Bacterial expression of hnRNPA2 truncation (residues 190 to 302).

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His tag and TEV

Expression

Bacterial

Mutation

contains amino acids 190-302

Promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_P298L_S285C

Plasmid

#104469

Purpose

express His tagged P298L S285C hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

P298L S285C

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD 11W D262V

Plasmid

#234618

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_11W D262V (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

F202W, F210W, F216W, F222W, F228W, Y237W, Y244W, …

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA1-FLAG-IRES-eGFP

Plasmid

#234619

Purpose

Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1 (HNRNPA1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal FLAG tag, co-expressing with eGFP via …

Promoter

CMV

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 201 - 240 of 407 results

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