Addgene

We narrowed to 10,856 results for: Kars;

Showing: 101 - 120 of 10856 results

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Cyto-ABKAR

Plasmid

#61510

Purpose

Expression of AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4

Plasmid

#61619

Purpose

FRET-based biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4

Use

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAR1A

Plasmid

#23741

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1A (PRKAR1A Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sapphireCKAR

Plasmid

#118468

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Use

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-blueCKAR

Plasmid

#118472

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512g3.1

Plasmid

#171200

Purpose

Expresses Br512g3.1 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Use

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512g3.2

Plasmid

#171201

Purpose

Expresses Br512g3.2 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Use

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Use

Tags

Citrine and ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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KARS_pNIC-Bio3

Plasmid

#153051

Purpose

Expression construct for producing Avi tagged Lysyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

KARS (KARS1 Human)

Use

Tags

Avi and His6-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR2-CR

Plasmid

#40255

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

AKAR2

Use

Tags

His6 and Xpress

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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EKARet-nuc

Plasmid

#96896

Purpose

FLIM sensor for ERK activation in nucleus

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for ERK Activity in Nucleus

Use

Tags

EGFP and sREAChet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAR1B

Plasmid

#23376

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1B (PRKAR1B Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-ABKAR

Plasmid

#140051

Purpose

Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-blueAKAR

Plasmid

#118470

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Use

Tags

Lamp1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

NLS (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only