Addgene

We narrowed to 1,125 results for: cas12

Showing: 101 - 120 of 1125 results

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pET47b-T7-dAsCas12f

Plasmid

#204641

Purpose

Expression of an inactive AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

inactive AsCas12f

Use

Tags

6xHis

Expression

Bacterial

Mutation

D225A

Promoter

T7

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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UAST-LbCas12a

Plasmid

#140621

Purpose

Gal4 dependent expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAST

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-LbCas12a

Plasmid

#213743

Purpose

For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ-Cas12j2

Plasmid

#173924

Purpose

Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

NLS-Cas12j2-NLS

Use

CRISPR

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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LbCas12a-DR-cr in PBCSK-

Plasmid

#126641

Purpose

Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Cas12c-sgRNA_BbsI_CAM

Plasmid

#183221

Purpose

Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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enAsCas12a-mSA

Plasmid

#182954

Purpose

Express enAsCas12a-mSA

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

enAsCas12a-mSA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ289 (ErCas12a)

Plasmid

#138126

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

ErCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e KRAB

Plasmid

#188506

Purpose

Expresses FLAG tagged PlmdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-KRAB-NLS

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ239A (dFnCas12a)-TV

Plasmid

#136379

Purpose

CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

dFnCas12a-TV

Use

CRISPR

Tags

Expression

Plant

Mutation

D917A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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BPK2014-AmCas12b

Plasmid

#121951

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b

Use

Tags

10xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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dCas12j3 VPR

Plasmid

#188517

Purpose

Expresses FLAG tagged dCas12j3 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j3 KRAB

Plasmid

#188507

Purpose

Expresses FLAG tagged dCas12j3 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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dCas12j2 VPR

Plasmid

#188518

Purpose

Expresses FLAG tagged dCas12j2 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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BPK2013-AkCas12b

Plasmid

#121950

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Use

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FnoCas12a-DR-cr in PBCSK-

Plasmid

#126642

Purpose

Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracr_LbCas12a.DR

Plasmid

#155049

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracr_AsCas12a.DR

Plasmid

#155050

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ293 (BhCas12b_v4)

Plasmid

#136383

Purpose

Gateway entry clone for BhCas12b_v4

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BhCas12b_v4

Use

CRISPR

Tags

Expression

Plant

Mutation

K846R/S893R/E837G

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ-Un1Cas12f1

Plasmid

#205143

Purpose

Un1Cas12f1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

Insert

Un1Cas12f1

Use

CRISPR

Tags

NLS

Expression

Mutation

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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